A new ratiometric fluorescence detection of heparin is reported with the ensemble of 1 and 2. This method is based on the respective ACQ (aggregation-caused quenched emission) and AIE (aggregation-induced emission) features of anthracene and tetraphenylethene. DLS, CLSM and fluorescent spectral investigations suggest that the variation of the fluorescence intensity ratio I(497)/I(421) is due to...

The purpose was to study the toxicity of cadmium (Cd) and to explore its potential to generate nanoparticles during detoxification. In order to demonstrate this, in vivo fluorescence imaging, X-ray diffraction, and flow cytometry were performed. The in vivo imaging showed a fluorescence signal after Cd treatment (CdCl2, 1.50 mg/Kg, intraperitoneally). By contrast, the control-rat fluorescence w...

Charged particles of extensive air showers (EAS), mainly electrons and positrons, initiate the emission of fluorescence light in the Earth’s atmosphere. This light provides a calorimetric measurement of the energy of cosmic rays. For reconstructing the primary energy from an observed light track of an EAS, the fluorescence yield in air has to be known in dependence on atmospheric conditions, li...

a simple, rapid, selective and highly sensitive fluorimetric method for determination of two catecholamines, i.e. norepinephrine (ne) and epinephrine (ep), in serum samples was developed. the method is based on the fluorescence sensitization of terbium (tb3+) by complexation with both catecholamines in the presence of lanthanum (la3+), as a co-cation, and in a na2so3 solution (chemical deoxygen...

Changes in molecular states of p-dimethylaminobenzonitrile (DMABN) coground with β-cyclodextrin (β-CD) were examined using solid-state fluorescence measurements. Formation of a DMABN/β-CD inclusion complex by coprecipitation was confirmed by powder X-ray diffraction measurement. The powder X-ray diffraction pattern of the ground mixture was a halo pattern and differed from the pattern of the mi...

The adenosine derivative N6,N6-dimethyladenosine (DMA) shows dual fluorescence in solvents of different polarity. In addition to the “normal” fluorescence at 330 nm, another band is observed at 500 nm. The long wavelength emission dominates in aprotic solvents but is dynamically quenched by protic solvents. Steady-state and lifetime measurements show that the emissions originate from two excite...

Recent studies on the newly cloned red fluorescence protein DsRed from the Discosoma genus have shown its tremendous advantages: bright red fluorescence and high resistance against photobleaching. However, it has also become clear that the protein forms closely packed tetramers, and there is indication for incomplete protein maturation with unknown proportion of immature green species. We have ...

Fluorescence microscopy with an improved contrast for fluorescence images is developed using an optical interference mirror (OIM) slide, which can enhance the fluorescence from a fluorophore as a result of the double interference of the excitation light and emission light. To improve the contrast of a fluorescence image using an OIM slide, a linearly-polarized excitation light was employed, and...

The systematic description of the complex photophysical behaviour of pyrene in surfactant solutions in combination with a quantitative model for the surfactant concentrations reproduces with high accuracy the steady-state and the time resolved fluorescence intensity of pyrene in surfactant solutions near the cmc, both in the monomer and in the excimer emission bands. We present concise model eq...

The emission anisotropy (EA) of 4-Dimethyl-amino-4'nitrostilbene (DNS), 4-Amino-4'-nitrostilbene (ANS) and 2,5-Di(4-biphenylyl)oxazole (BBO) was observed to be decreasing with increasing dielectric constant e of the solvent. The effect is interpreted as an influence of the Onsager reaction field on the volume of the solvent shell, which in the excited state is smaller in solvents with larger e....