considered the central issue of developmental biology. Most embryologists have been drawn to the field by the Whether studied at the tissue, cell, or molecular level, beauty of the developing embryo and the mystery of progress requires reliable knowledge of two closely reits structure emerging from a single fertilized egg. The lated topics: the fate map of the embryo and the cell classic public...

Abstract Study question Do embryo transfer (ET) procedural parameters affect the clinical outcome, in terms of positive human chorionic gonadotropin (hCG), pregnancy and live-birth? Summary answer Observation ET parameters, either as single or combined a model, does not seem to have predictive value on cycle outcome. What is known already It has been reported that several technical nature may i...

Abstract Study question Can deep learning models using time-lapse images of embryo development be used to predict ploidy and provide supplemental information embryologists for clinical decision-making? Summary answer We developed a general MDBS-Ploidy model that uses maternal age quality scores status. What is known already Ploidy status, or the presence lack chromosomal abnormalities, an impor...

Abstract Study question Can CHLOE-EQ, an AI embryo assessment support tool, automatically identify the optimal time-range of morphokinetic events where chance euploidy is maximized? Summary answer Embryos that are within normal range have increased chances being euploid compared to embryos developing at a pace outside range. What known already The introduction time-lapse technologies in IVF has...

Abstract Study question What can we learn from 3D reconstructions of cleavage-stage embryos derived Hoffman modulation contrast (HMC) time-lapses? Summary answer A simple spatial biomarker extracted embryo at the t4 and t8 stages is associated with blastulation, blastocyst quality, pregnancy live birth. known already Several works have demonstrated significant associations between cell arrangem...

Abstract Study question Is it beneficial to culture 1PN embryos for clinical use? Summary answer Culturing blastocyst potential use is all patients, especially those with low prognosis. What known already are only a single pronucleus visible at the time of fertilisation check. It generally assumed that such haploid, having 23 chromosomes in each their cells. Given haploid not viable, current gu...

Abstract Study question Does the TLI have an added value in reproductive outcome when separating system’s two components, undisturbed culturing, and morphokinetic embryo grading? Summary answer The system itself does not a significant value. More advanced algorithms should be applied to aid selection process. What is known already technology has been widely used for over decades culturing. A Co...

Abstract Study question Does blastocysts’ iDAScore® differs when ICSI is performed instead of IMSI in case teratozoospermia on the day vitro fertilization (D0)? Summary answer Blastocysts’ unchanged or at (D0). What known already In routine practice, for D0-teratozoospermia sometimes not because its higher cost and/or laboratory workload. Time-Lapse studies have shown that (IVF) using suboptima...

Background: In this consensus-based position paper, the scientific societies Fondazione Italiana Linfomi (FIL) and Società della Riproduzione Umana (SIRU) reviewed main aspects identified optimal paths aimed to preserve monitor fertility in patients diagnosed with lymphoma. Methods: The multi-disciplinary working group for paper was composed by 5 onco-hematologists, 4 gynecologist or andrologis...

Abstract Study question Is it possible to amplify whole mitochondrial DNA (mtDNA) and detect all mtDNA mutation in human embryo from spent media after culture? Summary answer We were able 43% of medium 24-hour culture. The most could be detected. What is known already Preimplantation genetic testing for aneuploidy (PGT-A) with biopsy now commonly used improve pregnancy rate. However, invasive r...