نتایج جستجو برای: embryoid body
تعداد نتایج: 733745 فیلتر نتایج به سال:
Control of self-renewal and differentiation of human ES cells (hESCs) remains a challenge. This is largely due to the use of culture systems that involve poorly defined animal products and do not mimic the normal developmental milieu. Routine protocols involve the propagation of hESCs on mouse fibroblast or human feeder layers, enzymatic cell removal, and spontaneous differentiation in cultures...
The signal transducer Stat5 plays a key role in the regulation of hematopoietic differentiation and hematopoietic stem cell function. To evaluate the effects of Stat5 signaling in the earliest hematopoietic progenitors, we have generated an embryonic stem cell line in which Stat5 signaling can be induced with doxycycline. Ectopic Stat5 activation at the point of origin of the hematopoietic line...
Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid bo...
Epidermal growth factor-like domain 7, Egfl7, is a largely endothelial restricted gene which is thought to have a role during the differentiation of embryonic stem cells (ESCs) along the endothelial lineage. While it has been shown that Egfl7 knock-down in zebrafish impairs endothelial cord formation, the role of the gene in mammals has been unresolved. Interpretation of mouse knockout studies ...
background: since embryonic stem (es) cells have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, es cells could potentially provide an unlimited cell supply for human transplantation. objective: in order to study the differentiation of mouse embryonic stem (mes) cells, they were cultured in suspension by using es media without leukemia inhibitory facto...
BioTechniques 349 Embryonic stem (ES) cells were established from the inner cell mass of blastocysts and have been shown to spontaneously differentiate into multiple cell types representative of all three embryonic germ layers, both in teratomas after grafting or in vitro under appropriate conditions (1,2). ES cells can be maintained in their pluripotent state if cultured in the presence of leu...
Background/Purpose: The aim of this study was to search for the optimum conditions for differentiating murine induced pluripotent stem cells (iPSCs) into contracting smooth muscle, as a precursor to utilizing in vitro tissue engineering techniques to generate functioning intestinal tract wall for patients with short bowel syndrome. Method: iPSCs were cultured for 6 days in low adherent 96 well-...
We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCKi/+spin, and -ROCKi/-spin. Cell suspensions of BG01V/hOG and H9 hESC lines were pipetted into non...
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