this experiment was carried out to study the effects of different levels of canola meal of different fat sources on the egg quality, performance as well egg yolk cholesterol during the first 12 weeks of age in laying hens (hy-line w-36). throughout the experiment a total of 432 hens were employed in a completely randomized design in a factorial arrangement of 3×4, with three levels of canola me...

A study was conducted to evaluate the effects of two laying systems (floor versus cage) on egg production, egg quality, and microbial safety. One hundred and eighty 42 wk old laying hens were separated into two groups of 90 hens each, and housed in laying cages and a floor laying system. Eggs from the hens were collected for 2 weeks, and hen-day egg production, egg quality (whole egg, albumen, ...

Schmallenberg virus (SBV), discovered in Europe in 2011, causes mild transient disease in adult ruminants, but fetal infection can lead to severe malformation in cattle, sheep and goats.To elucidate the pathogenesis of this novel orthobunyavirus, considerable efforts are required. A reliable and standardized infection model is essential for in vivo studies. In the present study, two groups of f...

The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate and vertebrate cells (invertebrate ↔ vertebrate) to simulate natural alternation and...

Cell culture Cell culture techniques have been described previously. C1 hiPSCs and the hESC lines WIBR#3 were maintained on mitomycin C inactivated mouse embryonic fibroblast (MEF) feeder layers in hESC medium [DMEM/F12 (Invitrogen) supplemented with 15% fetal bovine serum (FBS) (Hyclone), 5% KnockOutTM Serum Replacement (Invitrogen), 1 mM glutamine (Invitrogen), 1% nonessential amino acids (In...

Malaria parasites vary in virulence, but the effects of mosquito transmission on virulence phenotypes have not been systematically analysed. Using six lines of malaria parasite that varied widely in virulence, three of which had been serially blood-stage passaged many times, we found that mosquito transmission led to a general reduction in malaria virulence. Despite that, the between-line varia...

Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, respectively, under feeder- and serum-free culture conditions without enzyme. The hPSCs were detach...