Based on the adsorption of Fe(EDTA)- on a chitosan-coated glassy carbon electrode, a second-derivative square-wave voltammetry for the determination of the EDTA species in water samples was investigated. The measuring range of EDTA was from 6.0 x 10(-7) to 5.0 x 10(-5) mol/L with a correlation coefficient of 0.998 and a detection limit of 2.8 x 10(-7) mol/L. The relative standard deviation was ...

PURPOSE The present study was undertaken to detect metallo-beta-lactamase (MBL) in nosocomial isolates of Pseudomonas aeruginosa by four different phenotypic methods. METHODS Ninety-one consecutive P. aeruginosa isolates were subjected to susceptibility testing by disc-diffusion assay and Vitek 2. Imipenem resistance was determined by three different methods (disc-diffusion, Vitek 2 and E tes...

tests requiring EDTA tubes are or#{149} Day 0 represents the day that the sample was aliquoted and the aliquots were placed at the indicated dered simultaneously. Although we temperatures. Onginalvalues,obtained 0-5 days prior to aliquoting, were all the same as the day 0 value. speciQy that these specimens be shipped on solid 002, samples are often at ambient temperature during All samples wer...

Valinomycin, nigericin and trichlorocarbanilide were assessed for their ability to control the protonmotive force in Escherichia coli cells. Valinomycin, at high K+ concentrations, was found to decrease the membrane potential delta phi and indirectly to decrease the pH gradient delta pH. Nigericin was found to have two modes of action. At low concentrations (0.05-2 microM) it carried out K+/H+ ...

Controlled conversion of right-handed B-DNA to left-handed Z-DNA is one of the greatest conformational transitions in biology. Recently, the B-Z transition has been explored from nanotechnological points of view and used as the driving machinery of many nanomechanical devices. Using a combination of CD spectroscopy, fluorescence spectroscopy, and PAGE, we demonstrate that low concentration of l...

BACKGROUND α-Klotho is a protein mainly produced in the kidney. Its circulating form has been suggested to link renal damage and distant tissue pathology. As three assays to measure α-Klotho became commercially available, we performed an evaluation of these commercially available Klotho assays. METHODS We studied within-run variation, between-run variation, matrix effects, linearity, and reco...

Exposure to environmental and occupational toxicants is responsible for adverse effects on human health. Chelation therapy is the only procedure able to remove toxic metals from human organs and tissue, aiming to treat damage related to acute and/or chronic intoxication. The present review focuses on the most recent evidence of the successful use of the chelating agent ethylenediaminetetraaceti...

Objectives To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro. Materials and Methods Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethyl...

1. Glomerular filtration rate was determined from 'Cr-EDTA clearance in twenty hypertensive subjects treated with propranolol. 2. A significant mean decrease of 13% in the glomerular filtration rate was found during treatment, and an increase of the same order of magnitude after withdrawal of propranolol. 3. It is suggested that these changes are related to a reduction in the renal blood flow. ...

The intestinal permeability to markers was assessed in preruminant calves fed different milk substitutes containing skimmed milk powder or whey and soyabean products of differing antigenic activity as the protein sources. In Experiment 1, the 6 h urinary excretion of lactulose transiently decreased with the antigenic soyabean product but not that of sucrose of D-mannitol. In Experiment 2, the 6...