BACKGROUND Restructuring chromatin into morphologically distinct chromosomes is essential for cell division, but the molecular mechanisms underlying this process are poorly understood. Condensin complexes have been proposed as key factors, although controversial conclusions about their contribution to chromosome structure were reached by different experimental approaches in fixed cells or cell ...

Chromatin is a major nuclear component, and it is an active matter of debate to understand its different levels of spatial organization, as well as its implication in gene regulation. Measurements of nuclear chromatin compaction were recently used to understand how DNA is folded inside the nucleus and to detect cellular dysfunctions such as cancer. Super-resolution imaging opens new possibiliti...

In all organisms, DNA molecules are tightly compacted into a dynamic 3D nucleoprotein complex. In bacteria, this compaction is governed by the family of nucleoid-associated proteins (NAPs). Under conditions of stress and starvation, an NAP called Dps (DNA-binding protein from starved cells) becomes highly up-regulated and can massively reorganize the bacterial chromosome. Although static struct...

We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micelles, self-assembled in an aqueous solution. This synthetic approach yields size...

Biomass feedstocks are densified to facilitate easy handling, transportation and storage. Densification is a process of transforming loose biomass into highly densified form by the application of mechanical pressure. The mechanism involved in the process of densification is difficult to understand from existing information. A comprehensive understanding of the densification process for biomass ...

Dynamic regulation of chromosome structure and organization is critical for fundamental cellular processes such as gene expression and chromosome segregation. Condensins are conserved chromosome-associated proteins that regulate a variety of chromosome dynamics, including axial shortening, lateral compaction, and homolog pairing. However, how the in vivo activities of condensins are regulated a...

Additive manufacturing (AM) is enabling the fabrication of materials with engineered lattice structures at the micron scale. These mesoscopic structures fall between the length scale associated with the organization of atoms and the scale at which macroscopic structures are constructed. Dynamic compression experiments were performed to study the emergence of behavior owing to the lattice period...

To study the evolution of protoplanetary dust aggregates, we performed experiments with up to 2600 collisions between single, highly-porous dust aggregates and a solid plate. The dust aggregates consisted of spherical SiO2 grains with 1.5 μm diameter and had an initial volume filling factor (the volume fraction of material) of φ0 = 0.15. The aggregates were put onto a vibrating baseplate and, t...

The compression during tabletting is a complex and irreversible dynamic process and the study of it with instrumented tablet presses is beneficial for process and formulation optimization. The compaction and ejection cycle provides us suitable signals about the tabletting process. Instrumented presses used in tabletting research and development are normally equipped to measure punch force and d...

Biofouling of nanofiltration (NF) and reverse osmosis (RO) membranes for water treatment has been the subject of increased research effort in recent years. A prerequisite for undertaking fundamental experimental investigation on NF and RO processes is a procedure called compaction. This involves an initial phase of clean water permeation at high pressures until a stable permeate flux is reached...