Prolonged use of antiretroviral agents has been shown to reduce morbidity and mortality associated with human immunodeficiency virus (HIV) infection. However, the long term use of antiretroviral agents can result in adverse metabolic effects, including hyperlipidemia, insulin resistance, and diabetes. All of these metabolic consequences are risk factors for coronary artery disease and myocardia...

In the present study, a rapid fingerprint analysis of Ligusticum chuanxiong (L. chuanxiong) was developed based on ultra-fast liquid chromatography coupled with diode array detection (UFLC-DAD). The analysis time was shortened from approximately 75 min on conventional high-performance liquid chromatography (HPLC) to 40 min on UFLC. This method was validated in terms of stability [<4.40% relativ...

FeiYangchangweiyan capsule (FY capsule), a traditional Chinese medicinal preparation consisting of three medicinal herbs, has been used to treat bacterial dysentery, acute, and chronic gastroenteritis for several decades. In this study, a novel, convenient, accurate, and valid method was developed by using high-performance liquid chromatography (HPLC) coupled with diode array detection...

Crocins in commercial liquid saffron extracts (Saffr’activ®) were identified using high-performance chromatography (HPLC) with a diode array detector (DAD) and mass spectrometry (MS). The impact of storage on the qualities extract studied HPLC-DAD-MS by exposing trans-4-GG crocin to environmental factors. Light temperature induced degradation after only one week. Trans-4-GG was totally hydrolyz...

Address reprint requests to Dr. Jens D. Lundgren, DAD Coordinating Center, Copenhagen HIV Program, Section 044, Hvidovre University Hospital, 2650 Copenhagen, Denmark, or at [email protected] (Dr. Lundgren). *The members of the DAD study group are listed in the Appendix. N Engl J Med 2003;349:1993-2003. Copyright © 2003 Massachusetts Medical Society. background It remains controversial whether expos...

Your dad is lucky to have a son who is so concerned about his health. Encouraging a friend or family member to quit using an addictive substance can be difficult, so be patient if your dad doesn?t respond favorably right away. In addition to talking with your father about the health risks of chewing tobacco, you might also want to share with him some of the tobacco cessation resources listed be...

It is important for hydropower dams to estimate PMP (Probable Maximum Precipitation) appropriately and prepare the plan of facilities modification operational changes in advance, because future flood risk by climate change influence getting higher. In this paper, we compared three methods estimation, conventional method applying DAD (Duration Area Depth) analysis based on experienced rain resul...

Optimization of sample pre-treatment. Complete removal of cellular and further solid sample constituents, precipitation and removal of proteins, precipitation and removal of cholesterol from the blood sample and quantitative extraction of the analytes tryptophan, phenol, p-cresol and cholic acid in the diluted blood plasma phase. Step III: Combination and conversion of both isocratic HPLC/DAD/M...

Based on our recent finding that cardiac myosin binding protein C (cMyBP-C) phosphorylation affects muscle contractility in a site-specific manner, we further studied the force per cross-bridge and the kinetic constants of the elementary steps in the six-state cross-bridge model in cMyBP-C mutated transgenic mice for better understanding of the influence of cMyBP-C phosphorylation on contractil...

TGF-β/BMP (bone morphogenetic protein) signaling pathways play conserved roles in controlling embryonic development, tissue homeostasis, and stem cell regulation. Inhibitory Smads (I-Smads) have been shown to negatively regulate TGF-β/BMP signaling by primarily targeting the type I receptors for ubiquitination and turnover. However, little is known about how I-Smads access the membrane to execu...