A real-time reverse-transcription polymerase chain reaction (RT-PCR) was developed for efficient detection of genetically diverse Pepino mosaic virus (PepMV) isolates. The novel detection system was designed to use a duo-primer system targeting the conserved region in the triple gene block 2 (TGB2) gene with a single conserved TaqMan probe to broaden its reaction to cover all available PepMV st...

Four catheters (Ash Split Cath, Tesio, Duo-Split, and Duo--Flow; Medcomp, Harleysville, PA, U.S.A.) were tested in a temperature-controlled in vitro setup filled with 50% aqueous glycerin solution to determine hydraulic resistance at different flow rates. All these catheters have side holes; hydraulic resistance was determined with these holes open and closed. Due to extra pressure losses near ...

Huntington's disease (HD) occurs when the widely expressed protein huntingtin contains an expanded glutamine repeat. The selective degeneration and neuronal morphologic abnormalities of HD may involve interactions with proteins that bind to huntingtin, such as HAP1. The biological significance of this interaction is unclear because neither HAP1 nor huntingtin have significant homology to known ...

Male germ line development in flowering plants is initiated with the formation of the generative cell that is the progenitor of the two sperm cells. While structural features of the generative cell are well documented, genetic programs required for generative cell cycle progression are unknown. We describe two novel Arabidopsis (Arabidopsis thaliana) mutants, duo pollen1 (duo1) and duo pollen2 ...

In the Maximum Duo-Preservation String Mapping problem we are given two strings and wish to map the letters of the former to the letters of the latter as to maximise the number of duos. A duo is a pair of consecutive letters that is mapped to a pair of consecutive letters in the same order. This is complementary to the well-studied Minimum Common String Partition problem, where the goal is to p...