Thymidylate synthetases of human and bacterial origin form a tightly bound complex with the substrate dUMP in the presence of pteroyltriglutamate. This complex and the weaker enzyme . dUMP binary complex can be isolated and conveniently assayed by nitrocellulose disc filtration using [6-3H]dUMP as the radioactive ligand. Intact thymidylate synthetase . dUMP . pteroyltriglutamate complex can be ...

Heavy equipment is a vital resource in project, but renting heavy costly undertaking. Besides that, project always has constraints that allow the completion time to exceed specified time, and delays will also cause costs incurred increase. So from calculation of productivity equipment, it necessary so tool can work optimally. From analysis results, was found excavators 154.185 m3/Hour. For dump...

Stock ROM merupakan tempat pengumpulan sementara batubara hasil loading dari pit sebelum menuju crusher kemudian ke stockpile. Dalam penelitian desain stock di maksudkan untuk mengakomodasi jumlah target produksi yang telah direncanakan oleh PT Baturona Adimulya yaitu sebanyak 50.000 ton, dengan 25.000 langsung pelabuhan sedangkan sisanya akan ROM. mendesain sebuah diperlukan parameter – rancan...

In order to extract copper metal from the waste dump of Dexing copper mine and resolve the environmental problems caused by acidic water and heavy metals, a dump bioleaching plant was designed based on a series of experimental investigations. The investigation shown that the low-grade of the dump, refractoriness of chalcopyrite, leakage of pad, small Acidithiobacillus population and low dump pe...

This study deals with the control methods for automating operation of a hydraulic excavator loading soil onto bed dump truck. The requires moving bucket to target position without contacting In addition, there is demand minimize spillage during process. We applied Model Predictive Control (MPC) using constraints and objective functions corresponding requirements problem. To achieve desired beha...

This article considers one of the approaches to solving problem improving efficiency functioning unmanned open pit transport. The actual data on movements robotic dump trucks within framework a continuous transport and technological cycle at mining sites coal mine are analyzed. During study, movement times in loaded empty states recorded. In addition, time passing by individual sections route i...

The hybrid electric dump truck is equipped with multiple power sources, and each powertrain component controlled by an energy management strategy (EMS) to split the demanded power. This study proposes EMS based on deep reinforcement learning (DRL) algorithm extend battery life reduced total usage cost for vehicle, namely twin delayed deterministic policy gradient (TD3) EMS. Firstly, vehicle mod...

The Truck Factor designates the minimal number of developers that have to be hit by a truck (or quit) before a project is incapacitated. It can be seen as a measurement of the concentration of information in individual team members. We calculate the Truck Factor for 133 popular GitHub applications, in six languages. Results show that most systems have a small truck factor (46% have Truck Factor...

Due to the declining birthrate and aging population, shortage of labor in construction industry has become a serious problem, increasing attention been paid automation equipment. We focus on automatic operation articulated six-wheel dump trucks at sites. For trucks, it is important estimate position angle with high accuracy. In this study, we propose method for estimating state truck by using f...