Accurate prediction of in vivo hepatic drug clearance using in vitro assays is important to properly estimate clinical dosing regimens. Clearance of low-turnover compounds is especially difficult to predict using short-lived suspensions of unpooled primary human hepatocytes (PHHs) and functionally declining PHH monolayers. Micropatterned cocultures (MPCCs) of PHHs and 3T3-J2 fibroblasts have be...

Accurate prediction of in vivo hepatic drug clearance using in vitro assays is important to properly estimate clinical dosing regimens. Clearance of low-turnover compounds is especially difficult to predict using short-lived suspensions of unpooled primary human hepatocytes (PHHs) and functionally declining PHH monolayers. Micropatterned cocultures (MPCCs) of PHHs and 3T3-J2 fibroblasts have be...

Accurate prediction of in vivo hepatic drug clearance using in vitro assays is important to properly estimate clinical dosing regimens. Clearance of low turnover compounds is especially difficult to predict using short-lived suspensions of un-pooled primary human hepatocytes (PHHs) and functionally declining PHH monolayers. Micropatterned cocultures (MPCCs) of PHHs and 3T3-J2 fibroblasts have b...

Allopurinol (ALP) hypersensitivity is a major cause of severe cutaneous adverse reactions and is strongly associated with the HLA-B*58:01 allele. However, it can occur in the absence of this allele with identical clinical manifestations. The immune mechanism of ALP-induced severe cutaneous adverse reactions is poorly understood, and the T cell-reactivity pattern in patients with or without the ...

The confidential unit exclusion (CUE) option in the process to screen blood donors was conceived in the 1980s as a possible means to reduce the risk of transfusion transmission of HIV before sensitive tests became available. The use of CUE allows blood donors at increased risk for HIV or other sexually-transmitted diseases who may feel pressured to complete the donation, or who are inappropriat...

background and aims: human parvovirus b19, a member of the parvoviridae family, with single-stranded dna is a very minute non-enveloped virus. b 19 virus is mostly transmitted via the respiratory tract but some studies have been reported which b19 virus can be transmitted through blood and/or blood products. the aim of this study was to evaluate the prevalence of b19 among blood donors in tehra...

background: the differentiation between acute and latent forms of the toxoplasma gondii ( t. gondii ) infection is still considered as a complicated issue. this study was aimed to elucidate the status of infection in the blood donors and the probable importance of blood transfusion in the transmission of the infection through detecting both immunological and genetic markers of acute and latent ...

background: human leukocyte antigens (hla) are polymorphic cell surface proteins. distribution of hla alleles vary among different racial and ethnic populations in unrelated stem cell registries. determination of hla allele frequencies in different ethnic groups is useful for population genetic analyses. materials and methods: based on data available from the iranian stem cell donor registry, h...

ObjectiveThe objective of this study was to investigate whether clinical metabolomics, which is increasingly applied in population-based and epidemiological studies, can be used provide analytical evidence exposures, such information useful strengthen and/or complement corresponding database entries, taking drug use as an example.Study design settingLiquid chromatography-mass spectrometry (LC-M...