Freeze-dried B.C.G. of British manufacture became available for experimental work in 1955. The main reason for introducing it was the need for a vaccine which would retain its potency under conditions of prolonged storage. On reconstitution for injection the vaccine should produce a uniform suspension with minimal clumping, resulting in a uniform viable bacterial count. These properties mean th...

The effect of sample preparation on measures of in vitro digestibility was studied using leaves of Calliandra calothyrsus. They were chopped fresh (FrCh), chopped and oven-dried at 658C (ChOD), chopped and freeze-dried (ChFD), oven-dried and ground (ODGr) or freeze-dried and ground (FDGr). Samples were analysed for in vitro dry matter and nitrogen digestibility with or without PEG addition to b...

Background. Close therapeutic drug monitoring of Cyclosporine (CsA) in transplant outpatients is a favourable procedure to maintain the long-term blood drug levels within their respective narrow therapeutic ranges. Compared to basal levels (C(0)), CsA peak levels (C(2)) are more predictive for transplant rejection. However, the application of C(2) levels is hampered by the precise time of blood...

There is an increasing interest in the use of dried blood spot (DBS) sampling and multiple reaction monitoring in proteomics. Although several groups have explored the utility of DBS by focusing on protein detection, the reproducibility of the approach and whether it can be used for biomarker discovery in high throughput studies is yet to be determined. We assessed the reproducibility of multip...

Based on newly obtained 28S rDNA sequences from Roselliniella atlantica and R. euparmeliicola sp. nov., the genus Roselliniella has to be placed in Hypocreales and not in Sordariales; however, the family placement could not be resolved from the sequences obtained. The mature ascospores are single-celled and brown, but young ascospores are hyaline and sometimes have a median septum. The new spec...

The effects of spray-dried animal plasma in milk replacer without or with the addition of additives containing fructooligosaccharides and spray-dried serum on health, growth, and intake of Holstein calves was measured in two 56-d experiments. In experiment 1, 120 calves were fed milk replacer containing 0 or 20% of crude protein as spray-dried bovine plasma for 42 d and 30 to 60 g/d of additive...