Lipid membrane fluorescent probes: 23-(dipyrrometheneboron difluoride)-24-norcholesterol (BODIPY-Chol), GM1 Ganglioside (Brain, Ovine-Ammonium Salt), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N(lissamine rhodamine B sulfonyl) (ammonium salt) (Rh-DPPE) were purchased from Avanti Polar Lipids. Cholera Toxin Subunit B (CTXB) conjugated with Alexa488 and Alexa647 (A488CTXB, A647-CTXB) and D...

BACKGROUND Specific interactions between sphingomyelin (SM) and cholesterol (Ch) are commonly believed to play a key role in the formation of rafts in the biological membranes. A weakness of this model is the implication that these microdomains are confined to the outer bilayer leaflet. The cytoplasmic leaflet, which contains the bulk of phosphatidylethanolamine (PE), phosphatidylserine (PS) an...

Degree of unsaturation in fatty acid molecules plays an important role in the formation of vesicles. Vesicle formation from C18 fatty acids with different amount of double bonds such as oleic acid, linoleic acid and linolenic acid with the incorporation of 1,2-dipalmitoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DPPE-PEG2000) have been examined by TEM. Critical v...

Iridium-catalyzed intramolecular [4 + 2] cycloadditions of diene-tethered alkynyl halides were investigated by using [IrCl(cod)](2) as catalyst, and dppe was found to be the most suitable phosphine ligand for the reaction. No oxidative insertion of the iridium into the carbon-halide bond was observed, and the reactions proceeded to provide the halogenated cycloadducts in good yield (75-94%). Th...

A series of paramagnetic di(aryl)alkynylphosphine oxides [PF6] featuring an open-shell [Fe(κ(2)-dppe)(η(5)-C5Me5)](+) endgroup were obtained by oxidation of their neutral Fe(II) parents 3a-c, themselves obtained in a simple and nearly quantitative fashion from the corresponding Fe(II) metallophosphines 1a-c. The new organometallic radicals were characterised by NMR and ESR and were shown to be ...

The possible interaction of DDT with the lipids dimyristoyl lecithin (DML), dipalmitoylphosphatidylethanolamine (DPPE) and tripalmitin (TP) was studied. The work was carried out on oriented films and crystalline powders of DDT-lipid mixtures at different molar ratios by X-ray diffraction techniques. The diagrams showed only the patterns of pure DDT and that of the corresponding lipid. It is con...

The role phosphine ligands play in the palladium(ii)-bis-phosphine-hydride cation catalysed hydrogenation of diphenylacetylene is explored through a PHIP (parahydrogen induced polarization) NMR study. The precursors Pd(LL')(OTf)(2) (1a-e) [LL' = dcpe (PCy(2)CH(2)CH(2)PCy(2)), dppe, dppm, dppp, cppe (PCy(2)CH(2)CH(2)PPh(2))] are used. Alkyl palladium intermediates of the type [Pd(LL')(CHPhCH(2)P...

The biosynthesis of cyclic terpenes from polyene precursors by cyclase enzymes is one of nature’s most elegant chemical transformations.[1] The ease with which nature creates complex molecular architectures from achiral precursors has motivated efforts to develop similarly powerful synthetic methodologies.[2] Alimited number of asymmetric methods have been developed,[3] including the notable Br...

Regioselective dimerization of terminal aryl alkynes to produce conjugated enynes has been achieved using FeCl3 and KO(t)Bu in the presence of either DMEDA or dppe. The reaction proceeds smoothly in toluene at 145 °C for 2 h to give the corresponding head-to-head dimers in good to excellent yields (54 to 99%) with high E-selectivity (67 : 33 to 83 : 17 E/Z). Both strongly electron-donating and ...