Adiponectin is exclusively expressed in adipose tissue and secreted from adipocytes, and shows anti-diabetic and anti-atherogenic properties. However, the precise transcriptional mechanism of adiponectin remains elusive. In this study, the 5' flanking promoter region of human adiponectin gene was analyzed using UCSC genome browser, and a 10 390-bp fragment, containing an evolutionally conserved...

Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requi...

Runx2 is essential for osteoblast differentiation and chondrocyte maturation. The expression of Runx2 is the first requisite step for the lineage determination from mesenchymal stem cells to osteoblasts. Although the transcript from Runx2 distal promoter is majorly expressed in osteoblasts, the promoter failed to direct green fluorescent protein (GFP) expression to osteoblasts. To find the regu...

Transcriptional enhancers in large gene complexes activate promoters over huge distances, yet little is known about the mechanism of these long-range interactions. We report that the promoter targeting sequence (PTS) from the Abdominal-B locus of the Drosophila bithorax complex facilitates the activity of a distantly located enhancer in transgenic embryos and that it restricts the enhancer to a...

Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of present study was to determine impact endurance training on expression angiogenesis-related genes cardiac tissue diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including (DT), sedentary (...

Introduction: Sungkai (Peronema canescens Jack.) plant had been used as an immune system enhancer. Aim: In this study, the effect of leaf extracts from 4 different fractions, namely n-hexane, ethyl acetate, butanol and residual water with 3 variations in doses 1,10 100 mg/kg bw on activity NK CD8+T cells male white mice that have exposed to SARS-Cov-2 virus antigen was investigated. Methods: Th...

We have analyzed Emx2 enhancers to determine how Emx2 functions during forebrain development are regulated. The FB (forebrain) enhancer we identified immediately 3' downstream of the last coding exon is well conserved among tetrapods and unexpectedly directed all the Emx2 expression in forebrain: caudal forebrain primordium at E8.5, dorsal telencephalon at E9.5-E10.5 and the cortical ventricula...

Transcription of the herpes simplex virus 1 (HSV-1) immediate early (IE) genes is determined by multiprotein enhancer complexes. The core enhancer assembly requires the interactions of the POU-homeodomain protein Oct-1, the viral transactivator alphaTIF and the cellular factor C1 (HCF). In this context, the C1 factor interacts with each protein to assemble the stable enhancer complex. In additi...

Abstract This study examined the effect of Zeolite soil amendment on performance (growth and yield) sweet corn.The experiment was carried out in rice fields KP. Mojosari, Mojosari District, Mojokerto Regency MK II 2021 (April to September 2021), with 10 fertilization treatments which are combination best improver dose corn plants, including treatment A (control), B (Urea 200 kg/ha, ZA 100 NPK 2...