We describe for the first time a protocol to purify to apparent homogeneity an in vitro-prepared complex of prostate-specific antigen (PSA) and alpha 1-antichymotrypsin (ACT) by using a combination of gel filtration and ion-exchange chromatography. The purity of the PSA-ACT complex was confirmed by gel electrophoresis and Western blot. The PSA-ACT complex was stable in the pH range 6.0 to 7.8; ...

A pocked-sized turbine flowmeter and spirometer device, integrated with an electronic diary card (EDC-spirometer, Micro Medical, U.K.), was tested with a mechanical calibrator, in an outpatient clinic and in the home situation. A screen pneumotachometer was used as flow and volume reference. Ten devices were tested; interdevice variability was small with a mean variation coefficient of 1.1% for...

We present Very Long Baseline Array phase-referenced measurements of the parallax and proper motion of two pulsars, B0919+06 and B1857–26. Sub-milliarcsecond positional accuracy was obtained by simultaneously observing a weak calibrator source within the 40′ field of view of the VLBA at 1.5 GHz. We discuss the merits of using weak close calibrator sources for VLBI observations at low frequencie...

We report an ELISA-type titertray assay for autoantibodies against the ganglioside GM1. Trays were coated with ganglioside GM1 and reacted with patients' sera; bound IgM was detected with rabbit antibody to human IgM. High-titer serum from a patient was used as calibrator, another patient's serum as the positive control, and the GM1-specific cholera toxin as the control for GM1 coating. Regress...

BACKGROUND Therapeutic drug monitoring of adalimumab (ADM) has been introduced recently. When no detectable ADM serum concentrations can be found, the formation of antidrug antibodies (ADA) should be investigated. A variety of assays to measure the occurrence of ADA have been developed. Results are expressed as arbitrary units or as a titration value. The aim was to develop a monoclonal antibod...

We study the influence of the assumption behind the use of the inverse Tully-Fisher relation: that there should be no observational cutoffs in the TF parameter log VM. It is noted how lower and upper cutoffs would be seen in a logH0 vs. “normalized distance” diagram. Analytical expressions, under the simplifying assumption of a normal distribution and the use of the correct TF slope, are derive...

This report summarizes some trends observed in drug dissolution testing, based upon the United States Pharmacopeia (USP) dissolution Apparatus Suitability Test results and the preliminary data obtained from an international collaborative study to assess the pharmaceutical quality of furosemide products in different countries. Based on the USP calibrator data submitted by the participants, repre...

The presence of antibodies (Abs) in hemophilia A patients can potentially influence the therapeutic qualities of factor VIII (fVIII) administration. Much work has been focused on the presence of inhibitory antibodies, whereas the quantitation of noninhibitory anti-fVIII antibodies has been largely undetermined. Our objective was to develop a sensitive and specific fluorescence-based immunoassay...

Effective May 5, 2003, Landsat-5 (L5) Thematic Mapper (TM) data processed and distributed by the U.S. Geological Survey (USGS) Earth Resources Observation System (EROS) Data Center (EDC) will be radiometrically calibrated using a new procedure and revised calibration parameters. This change will improve absolute calibration accuracy, consistency over time, and consistency with Landsat-7 (L7) En...