A rabbit antiserum against bovine pancreatic DNase A is used to study the immunological reaction of DNases I. As shown by double immunodiffusion, bovine pancreatic DNases A, B, C, and D are immunologically identical, so are DNases from bovine pancreas and parotid and from ovine pancreas. These DNases also behave similarly in immunotitration of DNase activity and all are tightly bound to the imm...

Generation of DNA fragments is a hallmark of cell apoptosis and is executed within the dying cells (autonomous) or in the engulfing cells (non-autonomous). The TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labelling) method is used as an in situ assay of apoptosis by labelling DNA fragments generated by caspase-associated DNase (CAD), but not those by the downstream DNase II. In th...

Maintaining protein conformational stability and integrity during formulation is critical for developing protein pharmaceuticals. Accordingly, high sensitivity differential scanning calorimetry (HSDSC) and Fourier transform (FT)-Raman spectroscopy were employed to assess conformational stabilities (thermal stability and folding reversibility) and structural integrities, respectively, for three ...

Dermatophytes are keratinophilic fungi and the causative agent of dermatophytosis in animals people. In pathogenesis this disease, enzymes such as DNase, gelatinase, lipase, keratinase, elastase, collagenase highlighted. This work aimed to verify production these by clinical environmental isolates dermatophytes. Environmental strains were obtained Vanbreuseghem technique (1952), using soil samp...

The distribution of desoxyribonucleic acid (DNA) and ribonucleic acid (RNA) within cells of various organs has been extensively studied and the subject has been reviewed recently (l-3). On the other hand the information concerning the localization of nucleodepolymerases (4) is very limited. Ribonuclease (RNase) has been reported in chicken erythrocyte nuclei (5) and in mitochondria of rat liver...

BACKGROUND Colicin E7 (ColE7) is one of the bacterial toxins classified as a DNase-type E-group colicin. The cytotoxic activity of a colicin in a colicin-producing cell can be counteracted by binding of the colicin to a highly specific immunity protein. This biological event is a good model system for the investigation of protein recognition. RESULTS The crystal structure of a one-to-one comp...

An 8-fold enhancement in the activity of a DNA-dependent DNA polymerase was found in extracts from germinating wheat (Triticum vulgare var. Florence) embryos, as compared to the activity found in extracts from ungerminated embryos. The enhancement of this activity during the first hours of germination is concomitant to the increase of a Dnase activity. The two activities could be separated and ...

cemchemotherapy (22, 27, 28), the recent work of Tnouet et a!. (5, 24, 25) using a daunomycmn-DNA compex activated by DNase is an important example. In particular, the novelty of this approach is not in the ar@zymaticrelease mechanism but in the endocytosis mechanism proposed by DeDuve et a!. (6). The high endocytotic activity, characteristic of many tu mom cells, allows the transport of the ma...

Seaweed surfaces harbor diverse epibiotic bacterial communities with functions related to morphogenesis, host health, and defense. Among seaweed holobionts, culturable strains can represent innovative sources of bioactive compounds enzymes. The global industrial demand for microbial enzymes is continually growing in order improve certain manufacturing processes new perspectives exploitation. In...

Bacterial biofilms are associated with 80-90% of infections. Within the biofilm, bacteria are refractile to antibiotics, requiring concentrations >1,000 times the minimum inhibitory concentration. Proteins, carbohydrates and DNA are the major components of biofilm matrix. Pseudomonas aeruginosa (PA) biofilms, which are majorly associated with chronic lung infection, contain extracellular DNA (e...