This study describes a capillary-based device integrated with the heating and cooling modules for polymerase chain reaction (PCR). The device consists of the reaction polytetrafluoroethylene (PTFE) capillary, the aluminum blocks, and is equipped with two cartridge heaters, a thermoelectric (TE) cooler, a fan, and some thermocouples for temperature control. The cartridge heaters are placed into ...

This paper presents the comparative study of three phase twenty five level diode clamped and cascaded H-bridge multilevel inverters. The comparison is made in respect of requirement of devices, quality of output voltage and reduction of total harmonic distortion at the multilevel inverter terminals. In this work multicarrier sinusoidal pulse modulation control methods of Phase disposition (PD-P...

EGSs (external guide sequences) are short antisense oligoribonucleotides that elicit RNase P-mediated cleavage of a target mRNA, which results in inhibition of gene expression. EGS technology is used to inhibit expression of a wide variety of genes, a strategy that may lead to development of novel treatments of numerous diseases, including multidrug-resistant bacterial and viral infections. Suc...

The National Bridge Stock of Iran consists of about 330,000 bridges, of which around 50% are older than 30 years. Since 2010, Iran Road Maintenance & Transportation Organization has started implementing a comprehensive Bridge Management System in order to manage this aged stock efficiently. To predict future conditions of bridge stock, a heuristic numerical method is presented. This methodology...

Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Recent studies suggest that Lsr2 is a regulatory protein involved in multiple cellular processes including cell wall biosynthesis and antibiotic resistance. However, the underlying molecular mechanisms remain unknown. In this article, we performed biochemical studies of Lsr2-DNA interactions and structure-functio...

During cytokinetic abscission, the endosomal sorting complex required for transport (ESCRT) proteins are recruited to the midbody and direct the severing of the intercellular bridge. In this issue, Christ et al. (2016. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201507009) demonstrate that two separate but redundant pathways exist to recruit ESCRT-III proteins to the midbody.

In a recent paper Borel and Serre proved the theorem: If 8 is a Lie algebra of characteristic 0 and 8 has an automorphism of prime period without fixed points f^O, then 8 is nilpotent.1 In this note we give a proof valid also for characteristic p^O. By the same method we can prove several other similar results on automorphisms and derivations. Our method is based on decompositions of the Lie al...

Com mercial stat ic strain indicators and signal conditioners vary considerably in their circuit details; and, although most of them are based upon some form of the Wheatstone bridge circuit, the bridge circuit is employed in differing ways in different instruments. Because of the many variations in instrument design, a completely general treatment of instrument nonlinearities is not practicabl...

DNA-mediated charge transport (CT) is exquisitely sensitive to the integrity of the bridging pi-stack and is characterized by a shallow distance dependence. These properties are obscured by poor coupling between the donor/acceptor pair and the DNA bridge, or by convolution with other processes. Previously, we found a surprising periodic length dependence for the rate of DNA-mediated CT across a...

Tumor suppressor p53 is a sequence-specific DNA-binding protein and its central DNA-binding domain (DBD) harbors six hotspots (Arg175, Gly245, Arg248, Arg249, Arg273 and Arg282) for human cancers. Here, the crystal structure of a low-frequency hotspot mutant, p53DBD(R282Q), is reported at 1.54 angstroms resolution together with the results of molecular-dynamics simulations on the basis of the s...