The physiological role of dihydroxyacetone synthase (DHAS) in Candida boidinii was evaluated at the molecular level. The DAS1 gene, encoding DHAS, was cloned from the host genome, and regulation of its expression by various carbon and nitrogen sources was analyzed. Western and Northern analyses revealed that DAS1 expression was regulated mainly at the mRNA level. The regulatory pattern of DHAS ...

Evidence is presented showing that the dissimilation of carbohydrate reserves in two strains of bakers' yeast, Saccharomyces cerevisiae, is a purely respiratory process. Endogenous respiration is KCN-labile. Our own experiments together with various accounts and data given in the literature show that the same "oxygen-transporting mechanism" functions in both endogenous and exogenous metabolism....

Insecticides containing organophosphate, chlorinated hydrocarbon, and carbamate were tested with bovine ruminal ingesta fractions. Rumen bacteria exposed to insecticide levels of 0 to 500 ppm in rumen fluid for 4 hr were inoculated into rumen fluid-starch feed extract medium. No apparent significant bacterial count inhibitions were noted. Also, when insecticides were used as carbon sources at c...

Still [1940] compared the triosephosphate dehydrogenase of Escherichia -coli with the corresponding enzyme in yeast, and Utter & Werkman [1941] similarly compared the enzymes aldolase and isomerase of E. coli with those in miiscle and yeast. This communication extends this line of investigation to the reactions involving phosphoglyceric acid. Embden, Deuticke & Kraft [1933] and Meyerhof & Kiess...

Glucose containing radioactive carbon has been utilized in the study of the metabolic distribution of the various carbon atoms during dissimilation by the following organisms: Leuconostoc mesenteroides, (Gunsalus and Gibbs, J. Biol. Chem. 194, 871, 1952); Pseudomonas saccharophila, (Entner and Doudoroff, J. Biol. Chem. 196, 853, 1952); Pseudomonas fluorescens (Lewis et al., J. Biol. Chem., 216,...

Hansson (2007) demonstrated that, given an OT grammar with the constraint schemas CORRC↔C and IDENT-CC-x, factorial typology calls for grammars where certain segments are opaque to long-distance consonant assimilation (LDCA). That is, it is possible to rank CORR-C↔C and IDENT-CC-x constraints such that, for example, in a language with place harmony for sibilants, a voiced [z] will be transparen...