1. Degradation of 125I-labelled [Ile5]-angiotensin I and unlabelled angiotensin I was studied during incubation of rat plasma treated with disodium ethylenediaminetetra-acetate (disodium EDTA) at 37 degrees C, pH 6.5, for 1 h with 8-hydroxyquinoline, phenylmethylsulphonyl fluoride or di-isopropyl fluorophosphate. 2. Isoelectric focusing showed approximately 100, 59--66 and 10--31% preservation ...

A new spectrofluorimetric method has been developed for the determination of adenosine disodium triphosphate (ATP). We studied the interactions between the doxycycline (DC)-Eu3+ complex and adenosine disodium triphosphate (ATP) by using UV-visible absorption and fluorescence spectra. Using doxycycline (DC)-Eu3+ as a fluorescence probe, under the optimum conditions, ATP could remarkably enhance ...

We investigated differences between goat and cow milk by exploring the factors influencing milk stability by testing the heat coagulation time. Heat-stable milk samples were characterized by a higher pH, a lower heating temperature, a lower calcium concentration, a higher concentrations of EDTA-2Na, phosphate and citrate. However the sucrose concentration caused different patterns of heat stabi...

The objective of the present study was to formulate and evaluate nasal delivery system contains Ondansetron hydrochloride 4 mg/ml. Ondansetron hydrochloride loaded colloidal dispersion were prepared for nasal drug delivery using various polymers like β-cyclodextrin, hydroxyl propyl βcyclodextrin at different concentrations. The optimized formulation contains 0.02% benzalkonium chloride as a pre...

The effect of in vivo administration of disodium ethan-l-hydroxy-1.1-diphosphonate (EHDP) on the mitogenic response of thymocytes or spleen cells were studied in young rats. When rats (2-3 weeks of age) were injected with EHDP (10mg/kg/day) subcutaneously for more than 6 days or injected once with EHDP (100mg/kg) subcutaneously or intraperitoneally, lipopolysaccharide-induced proliferative resp...

The olivocerebellar system has been proposed to function as a timing device for motor coordination in which inferior olivary neurons act as coupled oscillators that spontaneously generate rhythmic and synchronous activity. However, the inferior olive receives excitatory afferents, which can also drive the activity of these neurons. The extent to which the olivocerebellar system can intrinsicall...

Ethylenediamine-N,N,N’,N’-tetraacetic acid calcium (II) disodium salt (Ca (II)-EDTA) was investigated for its antibacterial activity against the periodontal pathogens Aggregatibacter actinomycetemcomitans, Prevotella intermedia and Porphyromonas gingivalis. We determined the minimum inhibitory concentrations (MIC) of Ca (II)-EDTA and its bactericidal activity. Ethylenediamine-N,N,N’,N’-tetraace...

From 2003 to 2005, deaths of 3 individuals as a result of cardiac arrest caused by hypocalcemia during chelation therapy were reported to the Centers for Disease Control and Prevention. Two were children, both of whom were treated with edetate disodium. At the time of this writing, the adult case was still under investigation. No previous cases of death resulting from hypocalcemia during chelat...

This study was performed to investigate the effects of adrenocorticoid steroids, Prednisolone sodium hemisuccinate, Dexamethasone, and Betamethasone disodium phosphate, on the wound healing of permanent pacemaker implantation, especially on the :findings of redness and local hemorrhage. It was found that prednisolone sodium hemisuccinate and betamethasone disodium phosphate had a remarkable pro...