Immature cotyledons of open-pollinated fruits from three sour cherry cultivars (Prunus cerasus L.) were excised and cultured on Murashige and Skoog medium supplemented with various combinations of auxin and cytokinin to induce somatic embryogenesis. Somatic embryogenesis occurred principally when using the combinations of 2,4-dichlorophenoxyacetic acid plus kinetin. Using a-naphthaleneacetic ac...

The ability to produce morphologically and developmentally normal embryos and, indeed, whole plants from undifferentiated somatic cells in culture, through the process of somatic embryogenesis, resides uniquely within the plant kingdom. Since the initial description of somatic embryo production from carrot callus cells more than 35 years ago (Steward et al., 1958), this unique developmental pot...

A spinach-supplemented diet exerts numerous health benefits, but high levels of oxalic acid and nitrate can cause medical problems, so their should be reduced, while the vitamins phytochemicals could further increased by breeding. Conventional spinach breeding is limited very complex sex determination. However, these limitations circumvented in synergy with a biotechnological approach. Accordin...

In plants, complete embryos can develop not only from the zygote, but also from somatic cells in tissue culture. How somatic cells undergo the change in fate to become embryogenic is largely unknown. Proteins, secreted into the culture medium such as endochitinases and arabinogalactan proteins (AGPs) are required for somatic embryogenesis. Here we show that carrot (Daucus carota) AGPs can conta...

bryogenesis is usefu1 for isolating genes expressed during embryogenesis, as embryegenic competence and the expressien of certain embryo-specific genes increases with increased stress. Fluorescent differential display (FDD) was perfonmed to isolate stress-induced genes in carrot somatic embryos, and five genes were isolated as embryonic tissue-specific genes. One ofthese genes was expressed unt...