Inhibitors of drug efflux pumps have great potential as pharmacological agents that restore the drug susceptibility of multidrug resistant bacterial pathogens. Most attention has been focused on the discovery of small molecules that inhibit the resistance nodulation division (RND) family drug efflux pumps in Gram-negative bacteria. The prototypical inhibitor of RND-family efflux pumps in Gram-n...

Pd- and Pt-bound bis-metalated peptides were synthesised by the condensation of Pd- or Pt-aldimine-complex-bound glutamic acids to afford the four possible metal isomers of bis-Pd and bis-Pt-homometalated dipeptides and PdPt- and PtPd-heterometalated dipeptides without metal disproportionation. Ultrasound-induced self-assembly of these bis-metalated peptides proceeded effectively to afford supr...

Proteins and peptides of housefly larvae (HFL) have potential applications in food therapy. The fate HFL proteins following human gastrointestinal (GI) digestion is unknown. This study adopted a computational approach to discover released from upon GI digestion. In silico eight major 783 peptides. comprised 243 exhibiting 13 types bioactivities. Ninety-two single-function anti-dipeptidyl peptid...

1. The activity of peptidase enzyme(s) was measured in homogenates of mucosa taken at slaughter from sites along the length of sheep small intestine. 2. The activity of the homogenate towards six dipeptides showed a similar pattern of distribution for each substrate. Activity was lowest in the proximal duodenum, rising through the jejunum to peak in the mid-ileum and declining towards the termi...

A quantitative perfusion method has been used to study intestinal absorption of two dipeptides-glycyl-glycine and glycyl-l-alanine-in normal subjects. In each case, the constituent amino acids were absorbed faster when presented as dipeptides than as free amino acids, suggesting intact dipeptide transport. During absorption constituent amino acids were measured within the lumen and it is sugges...

The molecular recognition properties of pseudopeptidic macrocycles have been studied by ESI-MS and NMR spectroscopy, as highly complementary experimental techniques in solution and in the gas phase. We used ESI-MS competition experiments for the high throughput screening of the supramolecular interaction between four macrocyclic receptors and different peptide-like substrates in solution, rende...

Carnosinases are Xaa-His dipeptidases that play diverse functions throughout all kingdoms of life. Human isoforms of carnosinase (CN1 and CN2) under appropriate conditions catalyze the hydrolysis of the dipeptides carnosine (β-alanyl-L-histidine) and homocarnosine (γ-aminobutyryl-L-histidine). Alterations of serum carnosinase (CN1) activity has been associated with several pathological conditio...

Although Porphyromonas gingivalis is known to utilize peptides preferentially, instead of free amino acids, as the source of energy and cell material, there is only limited information on what sizes and kinds of peptide this bacterium preferentially utilizes. In this study, therefore, we tested aspartate or glutamate monopolymers consisting of from 2 to 100 amino acids as metabolic substrates f...

Dry-cured ham-derived dipeptides, generated along a dry-curing process, are of high importance since they play role in flavor development dry-cured ham. The objective this study was to analyze the residues less-studied metabotropic glutamate receptor 1 (mGluR1) implicated recognition umami ham dipeptides by molecular docking simulation using AutoDock Suite tool. AH, DA, DG, EE, ES, EV, and VG (...