There appear to be several pathways in liver by which Dand n-glyceraldehyde can be converted to dihydroxyacetone phosphate and n-glyceraldehyde phosphate. Thus, the following reactions have been described in liver preparations: phosphorylation of n-glyceraldehyde to n-glyceraldehyde phosphate (Fig. 1; Pathway A) (2); formation of n-glyceraldehyde phosphate from n-glyceraldehyde via n-glyceric a...

We have found that 0-alkyl bonds in glycerolipids are synthesized by microsomal enzymes of preputial gland tumors when diiydroxyacetone-P or glyceraldehyde-3-P and 1 J4C long chain fatty alcohols are used as substrates; ATP, coenzyme A, and Mg++ are essential cofactors. The 0-alkyl moieties formed from the 1-“Cand 9,10-3H-labeled long chain fatty alcohols show that the fatty alcohol chains are ...

Class I fructose-1,6-bisphosphate aldolases catalyze the interconversion between the enamine and iminium covalent enzymatic intermediates by stereospecific exchange of the pro(S) proton of the dihydroxyacetone-phosphate C3 carbon, an obligatory reaction step during substrate cleavage. To investigate the mechanism of stereospecific proton exchange, high resolution crystal structures of native an...

Patients with the cerebrohepatorenal syndrome of Zellweger lack peroxisomes and certain peroxisomal enzymes such as dihydroxyacetone phosphate acyltransferase in their tissues. Deficiency of this enzyme, which is necessary for glycerol ether lipid synthesis, provides a biochemical method for recognizing patients with subtle manifestations of Zellweger syndrome and suggests the utility of exogen...

The kinetics of conversion of dihydroxyacetone (DHA) to methylglyoxal (MGO) were investigated in mānuka honeys and DHA-doped clover honeys stored between 4 and 37°C. Both the disappearance of DHA and appearance of MGO were confirmed as overall, first order reactions, albeit probably composites of multiple reactions. Increasing the storage temperature accelerated the rate of DHA loss and the ini...

3 Bonsignore, A., S. Pontremoli, E. Grazi, and M. Mangiarotti, Biochem. Biophys. Research Communs., 1, 79 (1959). 4 Venkataraman, R., and E. Racker, J. Biol. Chem., 236, 1876 (1961). 5 Prandini, B. D., and J. A. Lopes do Rosario, Boll. soc. ial. biol. sper., 36, 1224 (1960). 6 Grazi, E., M. A. Mangiarotti, and S. Pontremoli, in press. 7 Bonsignore, A., S. Pontremoli, G. Fornaini, and E. Grazi, ...

We have built a new isonucleoside derivative on a 2,6-dioxobicyclo[3.2.0]heptane skeleton as a potential anti-HIV agent. To synthesize the target compound, an acetal-protected dihydroxyacetone was first converted to a 2,3-epoxy-tetrahydrofuran derivative. Introduction of an azide group, followed by the formation of an oxetane ring, gave a pseudosugar derivative with a 2,6-dioxobicyclo[3.2.0]hep...