Tritium-labeled digitoxin, digitoxigenin, digoxin, and digoxigenin of established purity and chemcal authenticity were used to study the binding of these compounds to human plasma proteins. 97% of digitoxin in plasma was nondialyzable. Continuous flow paper electrophoresis of plasma containing digitoxin and dialysis experiments in which human serum albumin competed for the glycoside with plasma...

Previously, we described a technique for fluorescence in situ hybridization (FISH) in whole-mount Drosophila embryos using fluoresceinlabeled RNA probes (2). This technique provides several advantages over conventional alkaline phosphatasebased detection methods. For example, a fluorescent label is nondiffusible and so allows higher-resolution imaging. Furthermore, it allows the use of confocal...

HIV-1 DNA extracted from frozen and formalin fixed brain tissue can be detected using PCR. This work has been extended by amplifying, using semiquantitative PCR, HIV DNA extracted from frontal lobe tissue of 16 patients with AIDS (eight positive and eight negative for p24 antigen). DNA was amplified using HIV-1 pol gene digoxigenin labelled primers and detected by chemiluminescence and densitom...

We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesti...

In this colorimetric immunoassay for digoxin, large, unilamellar phospholipid vesicles approximately 0.2 micron in diameter are loaded with high concentrations of Sulforhodamine B. Digoxigenin coupled to phosphatidylethanolamine, incorporated into the lipid formulation, confers immunological specificity. The liposomes are then used as tracers in simple competitive-binding immunoassays with anti...

A polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of Fusobacterium nucleatum Fev1, coding for a part of the 40-kDa major outer membrane protein, was labeled with the steroid hapten digoxigenin. The probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. It was therefore superior in di...