Olive mill solid waste (OMSW) is a pollutant waste coming from olive oil elaboration by the two-phase centrifugation system. OMSW has a high organic matter content and unbalanced carbon to nitrogen (C/N) ratio, 31/1, which avoids obtaining high methane yields in the anaerobic digestion of this waste. In the present study a microalgae, Dunaliella salina, was employed as co-substrate for the OMSW...

The processes of anaerobic digestion and co-digestion are complicated and the development of computational models that are capable of simulation and prediction of anaerobic digester performances can assist in the operation of the anaerobic digestion processes and the optimization for methane production. The artificial neural network approach is considered to be an appropriate and uncomplicated ...

Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of larger 1D gel-derived gel bands with liquid handling work stations is less we...

To determine the importance of digestion and detrition (rubbing) in the rumen on the breakdown of large particles (LP) in the leaf and stem fractions of temperate and tropical forages, a study was made using a digestion-detrition simulator. Large-particle breakdown was measured in forage samples subjected to 48-h digestion or 48-h digestion plus detrition. Digestion caused a breakdown of 14% +/...

BACKGROUND Microwave is the most reliable sample digestion method. However, it requires expensive microwave digester automation and has relatively low productivity. In this study, three non-automated digestion methods, i.e. wet acid digestion using nitric acid (HNO3) and hydrogen peroxide (H2O2), wet acid digestion using HNO3, and dry washing, are compared in order to determine the best approac...

INTRODUCTION When feeding grains to feedlot cattle, the goal is to maximize starch digestion while managing acidosis in order to provide optimal economic utilization of the finishing diet. Starch, the main component of grains, is digested primarily in the rumen with some digestion occurring in the small intestine and cecum. The small intestine is the preferred location of starch digestion to oc...

In most of the world's population the ability to digest lactose declines sharply after infancy. High lactose digestion capacity in adults is common only in populations of European and circum-Mediterranean origin and is thought to be an evolutionary adaptation to millennia of drinking milk from domestic livestock. Milk can also be consumed in a processed form, such as cheese or soured milk, whic...

Susceptibility to RNase digestion has been used to probe the conformation of the hammerhead ribozyme structure prepared from chemically synthesised RNAs. Less than about 1.5% of the total sample was digested to obtain a profile of RNase digestion sites. The observed digestion profiles confirmed the predicted base-paired secondary structure for the hammerhead. Digestion profiles of both cis and ...

Tryptic digestion is an important preanalytical step in shotgun proteomics because inadequate or excessive digestion can result in a failed or incomplete experiment. Unfortunately, this step is not routinely monitored before mass spectrometry because methods available for protein digestion monitoring either are time/sample consuming or require expensive equipment. To determine if a colorimetric...