The current ongoing studies and elucidations of control mechanisms of stress tolerance at molecular level that might facilitate the usage of molecular tools for developing further tolerant floras, is mainly centered on the expression of specific genes related to stress. Differential Display Reverse Transcriptase polymerase chain reaction (DDRT-PCR), a competent, profound and reproducible techno...

Many protein-tyrosine phosphatases (PTPases) have now been identified, but little is known about PTPase expression and regulation in vascular tissue and in vascular disease. Polymerase chain reaction (PCR) amplification and cDNA fingerprinting of PTPase catalytic domains, combined with random sequencing of PCR product libraries, identified 18 (8 receptor-like and 10 cytosolic) PTPases in the ra...

background and objectives: jak2 is a nonreceptor tyrosine kinase that plays a major role in myeloid disorders. jak2v617f mutation is characterized by a g to t transverse at nucleotide 1849 in exon 12 of the jak2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the jak2 protein. in this study we compared two molecular methods ...

Currently, the gradual introduction and routine application of validated Y chromosomal STR systems in stain analysis is being experienced. These systems are powerful forensic tools especially for the area of sexual assault evidence and are superior to autosomal systems for two reasons: the differential cell lysis step prior to the PCR is replaced by a sex chromosome selection during PCR and the...

Methods Reconstituted human gingival epithelia (RHGE) were challenged with two isoforms of PgLPS together with E. coli LPS as the positive control. mRNA and proteins were harvested from tissues and culture supernatants were collected. Expression of pro-inflammatory and antiinflammatory cytokines was evaluated by Q-PCR and ELISA. Involvement of pattern recognition receptors and signaling pathway...

Background: To prevent the losses caused by brucellosis, development of diagnostic tools for differentiating vaccinated cattle from infected one is priority in eradication disease. The present study was undertaken differential identification Brucella abortus vaccine strain S19 EryC PCR. Methods: In this study, 1145 clinical samples unvaccinated and buffaloes (200 blood samples, 710 sera, 190 va...

Representational difference analysis (RDA) is a powerful technique for cloning the differences between genomes, and has recently been adapted for cloning differentially expressed genes. RDA, like other PCR-based differential screening methods, is prone to the production of false positives. We have identified a major source of false positives in RDA of cDNA and have introduced improvements which...

mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to ...

Aim: The urokinase-type plasminogen activator (uPA) system consists of a protease, receptor (urokinase-type receptor, uPAR), and inhibitors that can be expressed on various cell types. Previous literature shows the amount soluble (suPAR) secreted from affected cells is higher in Crimean-Congo hemorrhagic fever (CCHF) patients than healthy controls. Thus, we aimed to investigate diagnostic value...