نتایج جستجو برای: diagnostic serological assays

تعداد نتایج: 415011  

Journal: :Parasitology international 2016
Samson Muuo Nzou Yoshito Fujii Masashi Miura Matilu Mwau Anne Wanjiru Mwangi Makoto Itoh Md Abdus Salam Shinjiro Hamano Kenji Hirayama Satoshi Kaneko

Human African trypanosomiasis (HAT) is a disease caused by Kinetoplastid infection. Serological tests are useful for epidemiological surveillance. The aim of this study was to develop a multiplex serological assay for HAT to assess the diagnostic value of selected HAT antigens for sero-epidemiological surveillance. We cloned loci encoding eight antigens from Trypanosoma brucei gambiense, expres...

Journal: :Journal of clinical microbiology 2003
Zaher Zeaiter Pierre-Edouard Fournier Gilbert Greub Didier Raoult

Bartonella endocarditis is a severe disease for which blood cultures frequently remain negative. We tested three PCR assays by using specimens of serum sampled early during the disease from 43 patients diagnosed in our laboratory as having Bartonella endocarditis on the basis of serological, culture, and/or valvular molecular detection. We tested a two-step nested PCR (TSN-PCR), a one-step nest...

Journal: :Journal of clinical microbiology 2006
Elisa Beghetto Andrea Spadoni Luca Bruno Wilma Buffolano Nicola Gargano

We have evaluated the diagnostic utility of six antigenic regions of the Toxoplasma gondii MIC2, MIC3, M2AP, GRA3, GRA7, and SAG1 gene products, assembled in recombinant chimeric antigens by genetic engineering, in order to replace the soluble, whole-cell tachyzoite extract in serological assays. Serum samples from 100 adults with acquired T. gondii infection and from 30 infants born to mothers...

Journal: :Clinical Chemistry 2023

Abstract Background Serology testing of antibodies against SARS-CoV-2 viruses serves many purposes: disease diagnosis, on-going and surveillance, epidemiologic vaccine studies, etc. There was a critical need to provide standardized quality control materials for development harmonization across different test methods IVD platforms. The MAS™ IgG Controls are human plasma-based containing SARS-CoV...

Journal: :Antiviral therapy 2012
Bryce D Smith Amy Jewett Jan Drobeniuc Saleem Kamili

Approximately 4.1 million Americans have been infected with HCV and 45-85% of chronically infected persons are unaware of their status. Rapid anti-HCV assays can assist and expedite the identification of those unaware of their infection. Performance characteristics of pre-market rapid anti-HCV assays (Chembio, MedMira and OraSure) have been evaluated. Their sensitivity (78.9-99.3%) and specific...

Journal: :BMC Clinical Pathology 2008
Eskild Petersen Martin Tolstrup Francesco Capuano Svend Ellermann-Eriksen

BACKGROUND Testing for Borrelia-specific IgM and IgG-antibodies are often performed on a variety of poorly defined symptoms, and isolated IgM results are a frequent finding, which results in diagnostic uncertainty and further testing. We wanted to test the hypothesis that Borrelia-specific assays using recombinant antigens perform differently from assays based on purified flagella antigen. ME...

2016
Tanja Vollmer Juergen Diekmann Matthias Eberhardt Cornelius Knabbe Jens Dreier

Diagnosis of hepatitis E virus (HEV) is usually determined serologically by detection of the presence of immunoglobulin (Ig)M antibodies or rising anti-HEV IgG titers. However, serological assays have demonstrated a significant variation in their sensitivities and specificities. In this study, we present the systematic comparison of different immunological anti-HEV assays using complete serocon...

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