inhalation of pollens from different species of acacia is a common cause of respiratory allergy in tropical areas of the world. acacia farnesiana is commonly used as street trees in towns and ornamental shade trees in parks and gardens throughout arid and semi-arid regions of asia. this study aimed to produce and purify the a. farnesiana pollen profilin (aca f 2) and evaluate its nucleotide seq...

no abctract

background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

hemoglobin f (hbf, α 2 γ 2 ) is a major contributor to the clinical heterogeneity and ameliorating agent observed in patients with the β-globin disorders including β-thalassemia and sickle cell disease (scd). during fetal life, hbf is the major hemoglobin but is largely substituted by adult hemoglobin (hba, α 2 β 2 ) following a globin expression switch after birth. increased γ-globin expressio...

reteplase is a part of tissue plasminogen activator (t-pa) used for theremoval of thrombi in blood vessels. in the present study we express the reteplase genein escherichia coli top10 and then its thrombolytic activity was measured. the recombinant plasmid pbadgiiia was transformed into the competent escherichia coli top10 and then transformed bacteria was seeded into bioreactor containing 1.5 ...

introduction: regulators of g-protein signaling protein negatively control g protein -coupled receptor signaling duration by accelerating gα subunit guanosine triphosphate hydrolysis. since regulator of g-protein signaling4 has an important role in modulating morphine effects at the cellular level and the exact mechanism(s) of adrenalectomy-induced morphine sensitization have not been fully cla...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

for a long time, scientists have tried to describe disorders just by genetic or environmental factors. however, the role of epigenetics in human diseases has been considered from a half of century ago. in the last decade, this subject has attracted many interests, especially in complicated disorders such as behavior plasticity, memory, cancer, autoimmune disease, and addiction as well as neurod...

background and objectives: the linear epitopes of gp46-i, gp46-ii, gp21 and p19 are used in diagnosis of htlv-i/-ii infections. the aims of this study was to obtain high-level expression and purification of recombinant antigen (ra) containing these epitopes. large-scale preparation of such antigen probably worths for diagnostic purpose. materials and methods: the synthetic dna encoding ra was s...

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