Standardization of a fragmented dermatophyte mycelial inoculum free of conidia for use in a broth microculture antimycotic susceptibility testing system is described. Ten clinical dermatophyte isolates were grown in submerged broth cultures at 35 degrees C. The mycelia were harvested before conidia appeared and were fragmented to 10 to 50-micron segments with a Broeck ground-glass tissue grinde...

OBJECTIVE To determine the role and pattern of non-dermatophyte moulds as causative agents of onychomycosis. STUDY DESIGN Case series. PLACE AND DURATION OF STUDY Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from November 2009 to July 2010. METHODOLOGY Nail clippings and nail scrapings were obtained from abnormal looking nails with treatment and dete...

Currently, tumor necrosis factor alpha (TNF-alpha) inhibitors are widely used for many autoimmune disorders. However, they cause an immunocompromised status that sometimes leads to many cutaneous side effects including atypical infections. Herein, we report the first case of adalimumab-related Majocchi's granuloma.A 43-year-old Taiwanese male patient with chronic plaque-type psoriasis developed...

BACKGROUND Onychomycosis is a rare disorder in children. Few studies exist on the incidence or prevalence of onychomycosis in children. OBJECTIVE To examine the epidemiology of childhood onychomycosis in Iceland during the period 1982-2000. METHODS Results from all mycological samples taken from children in Iceland from 1982 to 2000 were examined. Information about the requesting physician,...

Infection of the keratinised tissues (skin, hair and nails) in man and animals by keratinophilic fungi (dermatophytes) results in dermatophytosis (also known as tinea or ringworm). As conventional laboratory procedures for the identification of dermatophytes are either slow or lack specificity, improved diagnostic methods are required. The application of nucleic acid amplification technology ha...

The treatment of onychomycosis has improved considerably over the past several decades following the introduction of the oral antifungals terbinafine and itraconazole. However, these oral agents suffer from certain disadvantages, including drug interactions and potential liver toxicity. Thus, there is a need for new topical agents that are effective against onychomycosis. ME1111 is a novel sele...

Chronic exposure to toxigenic molds in water-damaged buildings is an indoor environmental health problem to which escalating health and property insurance costs are raising a statewide concern in recent times. This paper reviews the structural and functional properties of mycotoxins produced by toxigenic molds and their interactive health implications with antifungal drugs. Fundamental bases of...

Molds are filamentous (fuzzy or dusty looking) fungi that occur in many feedstuffs including roughages and concentrates. Molds can infect dairy cattle, especially during stressful periods when they are immune suppressed, causing a disease referred to as a mycosis. Molds also produce poisons called mycotoxins that affect animals when they consume mycotoxin contaminated feeds. This disorder is ca...

Enhancedgene replacements inKu80disruptionmutantsofthe dermatophyte,Trichophytonmentagrophytes Tsuyoshi Yamada, Koichi Makimura, Tatsuya Hisajima, Yumiko Ishihara, Yoshiko Umeda & Shigeru Abe Teikyo University Institute of Medical Mycology, Teikyo University, Tokyo, Japan; Genome Research Center, Teikyo University, Tokyo, Japan; and Department of Alternative Medicine, Faculty of Health Care, Te...

Thirty-two clinical isolates of anthropophlic dermatophytes were examined for their capacity to produce antibiotics in liquid culture and on human stratum corneum in vitro. Antibiotics were detected and classified using agar diffusion bioassays and chromatographic analysis. Twenty-four isolates produced antibiotic substances in liquid culture filtrates; some strains produced more than one antib...