Effects of soy isoflavones, genistein and daidzein, on the hepatic gene expression profile and indices for lipid metabolism were compared in rats. In the first experiment (Expt. 1), animals were fed diets containing 2 g/kg of either genistein or daidzein, or a control diet free of isoflavone for 14 days. In the second experiment (Expt. 2), rats were fed diets containing 1 or 2 g/kg of genistein...

Lundbaek et al. estimated the ratio of the volume of the lipid and the decane annulus VH to the aqueous volume VA as VH/VA ≈ 0.001. The hydrocarbon/aqueous partition coefficient K gives the ratio of concentration of bioflavinoid in hydrocarbon cH to its aqueous concentration cA as cH/cA=K. Therefore, the ratio of the amount of bioflavinoid in the hydrocarbon region BH to the amount in the aqueo...

The roles of cytochrome P450 monooxygenases (CYPs) from Streptomyces spp. which are called the "treasure islands" for natural products for medicine and antibiotics are not well understood. Substrate specificity studies on CYPs may give a solution for elucidation of their roles. Based on homology sequence information, the CYP105D7 of a soluble cytochrome P450 known as heme protein from Streptomy...

BACKGROUND Phytoestrogens may be associated with a reduced risk of hormone dependent neoplasms such as prostate and breast cancers. We tried to determine the validity of the association between serum phytoestrogen concentrations and dietary habits obtained from a food frequency questionnaire used in the Japan Collaborative Cohort Study (JACC Study) for Evaluation of Cancer Risk sponsored by the...

The roles of isoflavones in the prevention of several hormone-dependent cancers and osteoporosis are of great interest. Despite many pharmacokinetics studies of the isoflavones, the actual types of conjugates circulating in the body and the position(s) of conjugation sites on the flavone skeleton are still uncertain because, in general, conjugated compounds in biological fluids have been evalua...

Daidzein and its glycoside form daidzin, are known to have potential health benefits and are metabolized to O‑desmethylangolensin (O‑DMA) and equol following consumption. In the current study, the antioxidant activity and cytotoxicity of O‑DMA, equol, daidzein and daidzin was investigated and their effects on HepG2 human hepatocelluar carcinoma cells were compared. For cytotoxicity assays, lact...

The soybean isoflavones, daidzein, genistein, and glycitein, were hypothesized to act as cholesterol-lowering components, separate from soy protein. Pure synthetic daidzein, genistein, or glycitein (0.9 mmol/kg diet) or a casein-based control diet was fed to groups of 10 female Golden Syrian hamsters for 4 weeks. Hamsters fed glycitein had significantly lower plasma total (by 15%) and non-HDL (...

Background: Daidzin (glycosidic isoflavone) bioconversion into daidzein (aglyconic increases its bioactivity. This can be carried out using fermentation, a fungus that has been widely used for making tempeh in Indonesia, Aspergillus oryzae. Objective: study aimed to validate the method of analysis and determine content A. oryzae-fermented edamame TLC densitometry. Method: Daidzein determination...

BACKGROUND Isoflavones from soybeans may provide some beneficial impacts on postmenopausal health. The purpose of this study was to compare the pharmacokinetics and bioavailability of plasma isoflavones (daidzein and genistein) after a single dose of orally administered soy beverage and soy extract capsules in postmenopausal Thai women. METHODS We conducted a randomized two-phase crossover ph...

Soybean cyst nematode (SCN, Heterodera glycines) is a chief plant–parasitic of soybean. Application synthetic chemical nematicides poses negative side effects to human health and the environment. Therefore, search for safe effective approach more relevant. This study evaluated Genistein Daidzein on physiological index soybean nematodes, individual morphology, reversal frequency, respiration, bo...