The mechanism-based reagent 5-fluoro-alpha-d-glucopyranosyl fluoride (5F alpha GlcF) was used to trap a glycosyl-enzyme intermediate and identify the catalytic nucleophile at the active site of Aspergillus niger alpha-glucosidase (Family 31). Incubation of the enzyme with 5F alpha GlcF, followed by peptic proteolysis and comparative liquid chromatography/MS mapping allowed the isolation of a la...

Accurate determination of the ages individual whales is key to developing effective conservation strategies for bowhead whale (Balaena mysticetus). Previous attempts develop reliable methods age this species have included using body length and baleen measurements, carbon cycling analysis, assessments corpora accumulation, aspartic acid racemisation (AAR; conversion L D enantiomers) measurements...

This article reports experimental data related to the research article entitled "Poly(malic acid-co-l-lactide) as a Superb Degradation Accelerator for Poly(l-lactic acid) at Physiological Conditions" (H.T. Oyama, D. Tanishima, S. Maekawa, 2016) [1]. Hydrolytic degradation of poly(l-lactic acid) (PLLA) blends with poly(aspartic acid-co-l-lactide) (PAL) and poly(malic acid-co-l-lactide) (PML) oli...

It is known that the requirement of microorganisms for certain growth factors is influenced by the nutritive medium in a manner analogous to the effect of dietary regimen of animals on their vitamin requirements. In this connection it was found by Williams and Fieger (1) that oleic acid could substitute for biotin in the growth of Lactobacillus casei under their experimental conditions. Axelrod...

Buchanan, B. B. (Duke University, Durham, N.C.), and Leo Pine. Relationship of carbon dioxide to aspartic acid and glutamic acid in Actinomyces naeslundi. J. Bacteriol. 89:729-733. 1965.-CO(2), which was essential for the fermentation of glucose to succinic acid by Actinomyces naeslundii, was also required for the synthesis of internal aspartic acid, for which the cells lack a permease.

Cathepsin E belongs to the third class of enzymes - hydrolases, a subclass of peptide bond hydrolases and a sub-subclass of endopeptidases with aspartic catalytic sites. Cathepsin E is an endopeptidase with substrate specificity similar to that of cathepsin D. In a human organism, cathepsin E occurs in: erythrocytes, thymus, dendritic cells, epithelial M cells, microglia cells, Langerhans cells...

D-aspartic acid is abundant in the developing brain. We have identified and cloned mammalian aspartate racemase (DR), which converts L-aspartate to D-aspartate and colocalizes with D-aspartate in the brain and neuroendocrine tissues. Depletion of DR by retrovirus-mediated expression of short-hairpin RNA in newborn neurons of the adult hippocampus elicits profound defects in the dendritic develo...