BACKGROUND The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. RESULTS Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image...

*Corresponding Author: [email protected] Abstract Carvacrol is a component of numerous aromatic plants which has been evaluated for substantial pharmacological properties. Although the carvacrol induced cytotoxicity has already been reported, but no such study have been made on human cervical cancer, HeLa and SiHa cells. Therefore, in the present study an attempt has been made to investigate t...

A PCR assay detecting Clostridium difficile toxin B gene in stool specimens was compared to the cytotoxicity assay as the reference standard for the diagnosis of C. difficile antibiotic-associated diarrhea (CDAD). Overall, 118 stool samples were tested. All of the specimens that were negative by the cytotoxicity assay (59 out of 118) were also negative by the PCR method (specificity of 100%). O...

Introduction: Fluoroquinolones are potent inhibitors of bacterial topoisomerase II. They can also inhibit eukaryotic topoisomerase, and may confer antitumoral properties. Method: In this study the antitumoral activity of a new series of N-substituted piperazinylfluoroquinolones against a panel of human tumor cell lines was determined by MTT assays. Results: Among the tested compounds N-[2- (5-b...

Background: Several pharmacological effects have been attributed to thymol. However, limitations such as low solubility in water, bioavailability, and high volatility limited its use. Objectives: The present study aimed prepare characterize thymol-loaded solid lipid nanoparticles (SLNs) improve the efficacy of Methods: Thymol-loaded SLNs were characterized by atomic force microscopy (AFM), diff...

The Bacillus Ca/meffe-Gi/ér/n-activated macrophage-induced marrow cytotoxicity assay and the tumor-induced mar row cytotoxicity assay were used to explore in vitro the simi larities and differences between the mechanisms by which activated macrophages and cancer cells destroy normal cells in vivo. 1,3-Bis(2-chloroethyl)-1-nitrosourea, an agent that alkylates DNA, RNA, and proteins, is an effec...

Abstract Medicinal plants contain phytochemical components of pharmaceutical importance, and Trema orientalis MeOH extracts are believed to have potential antioxidant cytotoxic properties. This investigation explores the phenolic, antioxidant, anticancer property methanol aerial parts T. . The total polyphenol content (TPC) flavonoid contents (TFC) were determined following standard methods. In...

This study was planned to explore the capability of nanoemulsions (NEs) consisting of CapryolTM 90 and oleic acid for the delivery of rapamycin (RAP). Permeability and cytotoxicity of RAP-loaded NEs were also inspected. Pseudo-ternary phase diagrams were created with oleic acid and CapryolTM 90 (as oil phase) and four surfactants and co-surfactants at various weight ratios (Rsm). Selected NEs f...