Acid phosphatase (AP) reaction is a specific cytochemical marker for T-cell lymphoblastic leukaemia (T-ALL). Twenty six cases of ALL were diagnosed as T-ALL employing cytochemical profiles including AP and sheep red blood cell rosettes. Three district patterns of AP reaction were observed. On one end of the spectrum, 100% of the positive blasts showed the classical dot-like reaction while on th...

We have previously shown that human polymorphonuclear leukocytes (PMNs) killed organisms belonging to both human biovars of Chlamydia trachomatis. However, the mechanism of destruction was still unclear. We therefore conducted an ultrastructural and cytochemical study to investigate the mechanism of chlamydial degradation. PMNs were inoculated with the trachoma serovar B (B/TW-5/OT) or with the...

Acute community-acquired bacterial meningitis (BM) requires rapid diagnosis so that suitable treatment can be instituted within 60 minutes of admitting the patient. The cornerstone of diagnostic examination is lumbar puncture, which enables microbiological analysis and determination of the cerebrospinal fluid (CSF) cytochemical characteristics. However, microbiological testing is not sufficient...

Acid phosphatase levels in pure lymphocytes from normal individuals were determined cytochemically on intact cells and spectrophotometrically on cell extracts. Good correlation was demonstrated between the results obtained with both methods. Biochemical assay showed that a normal range of 7.2 +/- 0.9 and 7.1 +/- 1.0 mMoles per 10(6) lymphocytes was obtained without and with tartrate inhibition,...

Tartrate-resistant acid phosphatase (TRAcP) has been an indispensible marker for hairy cell eukemia (HCL) for over two decades. However, the traditional TRAcP cytochemical stain cannot be performed effectively on sections of paraffin-embedded tissues that are important resources for histopathologic evaluation in diagnosis and treatment of HCL. Wide variation in expression of TRAcP activity by h...

Ultrastructural and cytochemical features of phagosome degradation were examined in the tapetal retinal pigment epithelium (RPE) of the opossum. Didelphis virginiana. The tapetal RPE cells of the opossum measure as much as 100 micrometers in thickness, and the phagosomes traverse these cells so as to occupy a narrow region along the basal border. Both ultrastructural and cytochemical observatio...

background: acrylamide (aa) is an important industrial chemical primarily. aa is also found in carbohydrate-rich foods that are prepared at high temperatures, such as french fries and potato chips. it is demonstrated that aa is a carcinogen and reproductive toxin and has ability to induce sperm damage. objective: the aim of this study was to observe the effects of aa on sperm parameters and eva...

Secretion of saliva has been studies on man and animals from the histological, cytological, and other viewpoints since H e i d en h a i n (1868) and Zimmer m a n n (1898). On the histological and cytological changes of the salivary glands produced by some reagents, Altmann (1894), A r i m a (1918); Takagi (1925) and many others have reported. It is not enough for understanding the function of s...

The primary, secondary, and final reaction products of the cytochemical staining method for alkaline phosphatase have been demonstrated by x-ray emission microanalysis. The advantages and limitations of the technique in measuring reaction products are discussed. Scanning x-ray emission analysis provides a rapid way of detecting and measuring the amounts of individual elements in cytochemical re...

phological changes nor a remarkable decline of the enzyme activities was observed. These results suggest that the prophylaxis with TJ-9 prevents the occurrence ofhepatic injury, and therapeutic treatment improves the pathological changes induced by D-Gal. Comparing the methods of [[:J-9 administration to the rats, intrapcritoneal administration was more eflbctive than oral on the hepatic injury...