نتایج جستجو برای: culture conditions

تعداد نتایج: 1095904  

2012
Júlia Santos Cecília Leão Maria João Sousa

The manipulation of nutrient-signaling pathways in yeast has uncovered the impact of environmental growth conditions in longevity. Studies using calorie restriction show that reducing glucose concentration of the culture media is sufficient to increase replicative and chronological lifespan (CLS). Other components of the culture media and factors such as the products of fermentation have also b...

2013
Sophie M. Morgani Maurice A. Canham Jennifer Nichols Alexei A. Sharov Rosa Portero Migueles Minoru S.H. Ko Joshua M. Brickman

Embryonic stem cells (ESCs) are derived from mammalian embryos during the transition from totipotency, when individual blastomeres can make all lineages, to pluripotency, when they are competent to make only embryonic lineages. ESCs maintained with inhibitors of MEK and GSK3 (2i) are thought to represent an embryonically restricted ground state. However, we observed heterogeneous expression of ...

2009
Woo-Sik Jo Yun-Ju Cho Doo-Hyun Cho So-Deuk Park Young-Bok Yoo Soon-Ja Seok

Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom. The optimum tempe...

Journal: :Clinics (Sao Paulo, Brazil) 2008
Ayumi Aurea Miyakawa Luis Alberto Oliveira Dallan Silvia Lacchini Thaiz Ferraz Borin Jose Eduardo Krieger

INTRODUCTION Saphenous vein grafting is still widely used to revascularize ischemic myocardium. The effectiveness of this procedure is limited by neointima formation and accelerated atherosclerosis, which frequently leads to graft occlusion. A better understanding of this process is important to clarify the mechanisms of vein graft disease and to aid in the formulation of strategies for prevent...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2006
Jean Lu Runhua Hou Carmen Jane Booth Shih-Hung Yang Michael Snyder

Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of c...

Journal: :Molecules 2015
Sachchida Nand Pandey Hunain Khawaja Yi-Wen Chen

Facioscapulohumeral muscular dystrophy (FSHD) is believed to be caused by aberrant expression of double homeobox 4 (DUX4) due to epigenetic changes of the D4Z4 region at chromosome 4q35. Detecting DUX4 is challenging due to its stochastic expression pattern and low transcription level. In this study, we examined different cDNA synthesis strategies and the sensitivity for DUX4 detection. In addi...

Journal: :The Journal of General Physiology 1944
Jack Myers

1. Chlorella pyrenoidosa has been grown in a continuous-culture apparatus under various light intensities provided by incandescent lamps, other conditions of culture being maintained constant. The harvested cells were analyzed for cell number, dry weight, nitrogen, and chlorophyll per unit cell volume. 2. Cell nitrogen and cell volume are parallel measures of cellular material over the range of...

Journal: :Journal of microbiological methods 2000
M C Hansen R J Palmer D C White

We have developed an anaerobic biofilm culture system. The system is inexpensive, simple to use and, unlike an anaerobic glovebox, requires no dedicated space. As a test of the system, Porphyromonas gingivalis was cultured under low oxygen (1-2 ppm) and under anaerobic conditions (</=0.1 ppm O(2)). In the presence of small amounts of oxygen, the organism attached and formed an initial biofilm o...

Journal: :Human reproduction update 2015
M Tong C A Viall L W Chamley

Sir, WethankDrsClark andLaskin for their interest in our recentlypublished systematic review of the effects of antiphospholipid antibodies on cultured placental cells in vitro.We agreewithmany of the authors’ comments and their suggestions for future investigations. However, in suggesting that trophoblast plugs prevent antiphospholipid antibodies accessing the placenta in early gestation, we be...

2006
Woo-Sik Jo Young-Hyun Rew Sung-Guk Choi Geon-Sik Seo Jae-Mo Sung Jae-Youl Uhm

Phellinus genus belonged to Hymenochaetaceae of Basidiomycetes and has been well known as one of the most popular medicinal mushrooms due to high antitumor activity. This study was carried out to obtain the basic information for mycelial culture conditions of Phellinus linteus, P. baumii, and P. gilvus. According to colony diameter and mycelial density, the media for suitable mycelial growth of...

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