Nowadays, there is a significant need for synthetic bone replacement materials used in bone tissue engineering (BTE). Rapid prototyping and especially 3D printing is a suitable technique to create custom implants based on medical data sets. 3D printing allows to fabricate scaffolds based on Hydroxyapatite with complex internal structures and high resolution. To determine the in vitro behaviour ...

Traditional microbiological methods of cultivation recover less than 1% of the total bacterial species, and the culturable portion of bacteria is not representative of the total phylogenetic diversity. Classical cultivation strategies are now known to supply excessive nutrients to a system and therefore select for fast-growing bacteria that are capable of colony or biofilm formation. New approa...

OBJECTIVES Microbiological laboratory procedures are involved in diagnosis and therapy control of progressive and refractory forms of periodontitis. In recent years techniques have been developed based on the detection of nucleic acids. The purpose of this study was to validate the commercially available micro-Dent(R) test which employs probes for A. actinomycetemcomitans, P. gingivalis, P. int...

A total of 150 fecal and water samples from four swine farms were tested for the presence of Salmonella enterica using different enrichment techniques as follows: (i) 92 fecal samples from nursery and farrowing barns at three swine farms were preenriched overnight in tryptic soy broth (TSB) at 37 degrees C followed by overnight enrichment in Rappaport-Vassiliadis 10 broth (RV10) at 42 degrees C...

The uncultured miscellaneous crenarchaeotic group (MCG) archaea comprise one of the most abundant microbial groups in the Earth's subsurface environment. However, very little information is available regarding the lifestyle, physiology, and factors controlling the distribution of members of this group. We established a novel method using both cultivation and molecular techniques, including a pr...

As the nature of the causative agent of yellow fever was unknown, it was necessary at the beginning of these experiments to formulate a spedal method of cultivation. The methods employed have been similar to those recommended 1 for the cultivation of Leptospira icterohcemorrhagice (Inada and Ido). Instead of the serum and citrate plasma of the rabbit or other animal, serum and plasma from nonim...

Regionalization of crop cultivation is the foundation of large-scale planting and a reasonable layout scheme, but current approaches mainly rely on qualitative analysis and heuristic methods, which cannot meet the progressively challenging demands. In this study, we propose a spatial clustering approach that consists of two steps. First, attributive clustering is applied on the indicators. Seco...

The production of hormones has been suggested to be one of the mechanisms by which plant growth-promoting rhizobacteria (PGPR) stimulate plant growth. To evaluate whether the free-living soil bacterium, Paenibacillus polymyxa, releases the hormone group cytokinins and, if so, their identity, the content of cytokinins in the growth media, before and after cultivation of this bacterium, was deter...

Rice is the staple food in Tamil Nadu and is grown in an area of 2.6 Mha with a production of 8.19 Mt and productivity of 3.2 t/ha. In the context of high water demand by rice farmers, any strategy that would produce higher rice yield with less water is the need of the day. One such system is “System of Rice Intensification” (SRI), which was developed by Fr. Henri de Laulanie in Madagascar in 1...