AIMS Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood components, and subsequently to investigate their basic biological properties. METHODS DPSC were cultivated in five different media based on α MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DP...

In this study, 520 cultivated and 14 wild accessions of black gram (Vigna mungo (L.) Hepper) were assessed for diversity using 22 SSR markers. Totally, 199 alleles were detected with a mean of 9.05 alleles per locus. Wild black gram showed higher gene diversity than cultivated black gram. Gene diversity of cultivated accessions among regions was comparable, while allelic richness of South Asia ...

We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP-F 14-1...

PURPOSE To investigate the correlation between floating versus adherent growth pattern of cultivated retinoblastoma (RB) cells from three patients with RB and their histopathologic features. METHODS RB cells from three Iranian patients (MM, NR, and MS) were cultivated in Dulbecco's modified eagle medium (DMEM) supplemented with 15% fetal bovine serum (FBS) for four weeks in each passage. Fres...

Members of the primary gene pool of the chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum and four of C. echinospermum grown in India were investigated using 100 SSR markers to analyze their genetic structure, diversity and relationships. We found considerable diversity, with a mean of 4.8 alleles per locus (ranging from 2 to 11); polymorphic information content r...

The rapid development of agricultural modernization in Hubei province has an influence on cultivated land resources variations to a certain degree. This paper used grey relational analysis method,combined with Hubei province cultivated land area decreased data within the year covering 2000 to 2010 and agricultural modernization development index data, then analyzed the influence relationship be...

Chinese ginseng (Panax ginseng Meyer) is a medicinally important herb and plays crucial roles in traditional Chinese medicine. Pharmacological analyses identified diverse bioactive components from Chinese ginseng. However, basic biological attributes including domestication and selection of the ginseng plant remain under-investigated. Here, we presented a genome-wide view of the domestication a...

Cultivated flax (Linum usitatissimum L.) is the earliest oil and fiber crop and its early domestication history may involve multiple events of domestication for oil, fiber, capsular indehiscence, and winter hardiness. Genetic studies have demonstrated that winter cultivated flax is closely related to oil and fiber cultivated flax and shows little relatedness to its progenitor, pale flax (L. bie...

UNLABELLED PREMISE OF THE STUDY The taxonomy of cultivated potatoes has been highly controversial, with estimates of species numbers ranging from 3 to 17. Ploidy level has been one of the most important taxonomic characters to recognize cultivated potato species, containing diploid (2n = 2x = 24), triploid (2n = 3x = 36), tetraploid (2n = 4x = 48), and pentaploid (2n = 5x = 60) cultivars. We...

BACKGROUND The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. METHODS Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differenti...