High-resolution 303.6 MHz (31)P NMR spectra have been obtained of perchloric acid extracts of Plasmodium berghei trophozoites, Toxoplasma gondii tachyzoites, and Cryptosporidium parvum oocysts. Essentially complete resonance assignments have been made based on chemical shifts and by coaddition of authentic reference compounds. Signals corresponding to inorganic pyrophosphate were detected in al...

Cryptosporidium parvum causes acute diarrhea in immunocompetent individuals and a severe life-threatening disease in immunocompromised individuals, including AIDs patients. No efficacious therapy for cryptosporidiosis has yet been reported. However, treatment of some patients with cryptosporidiosis with hyperimmune bovine colostrum has ameliorated or eliminated clinical symptoms. Consequently, ...

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 9...

This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler's diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase ...

The 2 main species of Cryptosporidium that infect humans are Cryptosporidium hominis and C. parvum. Here, multilocus fragment analysis of 3 microsatellite loci (ML1, ML2, and gp60) was used to subtype strains from sporadic cases of cryptosporidiosis in Wales and northwest England. Of 72 strains of C. parvum, 63 were typeable at all 3 loci, forming 31 subtypes. These strains formed 3 broad clust...

Neofusicoccum parvum and Rhizoctonia solani are fungal pathogens with an increasing incidence in young grapevine plants. In this study, the antagonistic potential of some strains genus Trichoderma isolated from against these was investigated at laboratory greenhouse levels. In-plate confrontation assays showed that selected could inhibit mycelial growth both taxa, being more effective N. parvum...

Milk of naturally infected animal species is considered an essential reservoir Toxoplasma gondii, Neospora caninum, and Cryptosporidium parvum. An extant survey was performed to measure the T. N. C. parvum incidence periodic frequency amongst milk samples. One-thousand one-hundred sixty raw 400 pasteurized samples were collected. used for DNA extraction. The nested-polymerase chain reaction app...

Background: The intestinal tract of human can be infected by protozoan parasites. In this short communication, the stool samples were collected from patients with diarrhea referred to Kut hospital, Iraq, and then the parasites (Giardia lamblia, Entamoeba histolytica, Cryptosporidium parvum) were considered for molecular identification. Methods: Stool samples were collected from 69 patients wit...

BACKGROUND Cryptosporidium parvum is one of the most important biological contaminants in drinking water that produces life threatening infection in people with compromised immune systems. Dairy calves are thought to be the primary source of C. parvum contamination in watersheds. Understanding the spatial and temporal variation in the risk of C. parvum infection in dairy cattle is essential for...

Severe depletion of thym us-derived lymphocytes does not reduce, and may significantly increase, the resistance of mice to syngeneic tumours, although it grossly impairs their ability to reject alio transplants. Injection of killed C. parvum causes further inhibition of tum our growth in T-cell deprived mice to an extent comparable to th a t resulting from the same dose of G. parvum in normal m...