An experimental model was established using the terminal ileum of the rat for characterizing and studying the effects of crude cell-free extract from Clostridium perfringens upon physiological and histological parameters involved in the transport process. Further work was done with the model system using purified enterotoxin (protein) from the cell extract. Using an in vivo perfusion technique ...

The methanol, ethanol and petroleum ether soluble crude and fractions extract of Gliricidia sepium and Spathodea campanulata leaf were examined for antibacterial activities, phytochemicals and possible sources of antioxidant. The antibacterial activity of the crude and fractions were carried out against nine clinical bacteria isolates using the agar well and disc diffusion methods respectively....

Curcumae Rhizoma (CR) has been used in traditional Chinese medicine for thousands of years as an anti-blood stasis and pain-alleviating agent. However, the effects of crude and vinegar-processed Curcumae Rhizoma (VCR) on CYP450 activities are poorly understood. In this study, a “cocktail” method was designed to assess CYP1A2, CYP3A1 and CYP2E1 enzymatic activities in vivo using theophylline, da...

Antibiotic resistance makes Antimierobial peptides (AMPs) agents an alternative for treatment of pathogenic diseases. They are isolated from various invertebrate animals, vertebrates and plants. The present study shows the electrophoretic pattern of protein and peptides from Vicia faba seed and reports our first attempt to study the antibacterial activity of Vicia faba seed extract. The crude e...

OBJECTIVE The present study was an attempt to study total phenolic content and antioxidant property of the crude ethanolic extract of the roots of Azadirachta indica (A. indica). MATERIALS AND METHODS To evaluate the antioxidant properties of the crude extract, some complementary test systems, namely DPPH free radical scavenging assay, reducing power assay, and ferrous ion chelating ability a...

The aim of the study was to evaluate the effect of crude aqueous extract of Boswellia serrata Roxb. oleo gum on urinary electrolytes, pH and diuretic activity in normal albino rats. Moreover, acute toxicity of the gum extract was assessed using mice. Albino rats were divided into five groups. Control group received normal saline (10 mg/kg), reference group received furosemide (10 mg/kg) and tes...

objective(s): to appraise the antihepatotoxic efficacy of ethanolic extract of operculum turpethum root on the liver of swiss albino mice.   materials and methods:   hepatic fibrosis was induced in adult male albino mice through intraperitoneal administrations of n-nitrosodimethylamine (ndma) at the concentration of 10 mg/kg body weight. the liver toxicity and therapeutic effect of the plant et...

The marine sponge Spongosorites halichondriodes, collected from Mumbai coastal area, was studied for presence of bioactive proteins. Sponge species was identified based on Spicules morphology. Crude protein from the sponge was extracted at the concentration of 5.3 mg/mL in methanol and 3.4 mg/mL in aqueous extract. Six bacterial species (Klebsiella pneumonia, Salmonella typhi, Staphylococcus au...

Aqueous crude extracts of a series of plant wastes (agricultural, wild plants, residues from sports activities (grass), ornamental residues (gardens)) from 17 different plant species representative of the typical biodiversity of the Iberian peninsula were investigated as new sources of peroxidases (EC 1.11.1.7). Of these, lentil (Lens culinaris L.) stubble crude extract was seen to provide one ...

Chromatographic separation of an ethyl acetate extract from Embelia schimperi led to the isolation of a new compound identified as 2,5-dihydroxy-3-methyl-1,4-benzoquinone (1) on the basis of spectroscopic and physical data. The plant's crude extract and pure compound 1 were assayed for in vitro antimicrobial activity against clinical strains of Salmonella spp., Proteus spp., Pseudomonas aerugin...