I describe an automated enzymatic procedure to quantitate creatine in both serum and urine. In this assay, which requires no pretreatment of the sample, creatine kinase (CK; EC 2.7.3.2) and pyruvate kinase (EC 2.1.7.40) are used as auxiliary enzymes and lactate dehydrogenase (EC 1.1.1.27) is used in the indicator reaction. CK is also used as the starting reagent. Data obtained with the present ...

BACKGROUND AND PURPOSE Creatine kinase-MB (CK-MB) increases in some patients with stroke, with no clear evidence of an acute coronary syndrome. Its elevations have been suggested to represent a biological marker for stroke-related myocardial injury. Troponin T has superior sensitivity and specificity to CK-MB in revealing minor myocardial injury. Therefore, we studied troponin T levels after st...

background delayed onset muscle soreness (doms) is the sensation of stiffness and pain in the muscles that occurs 24-48 hours after unaccustomed or strenuous exercise. objectives the aim of this study was to examine and compare the effect of vibration training (vt) on delayed onset muscle soreness. materials and methods twenty healthy non-athletic volunteers (female, age 20-30 years, body mass ...

the aim of the present study was to determine the relationship between histopathological lesions and tissue enzymes in chickens affected by marek's disease.five apparently healthy chickens(as control group)and 25 chickens affected by marek's disease were selected. after consideration of history and gross lesions, tissue samples were collected from kidney, liver, heart, ovary, pectoral...

The enzyme creatine kinase (CK) [EC 2.7.3.2] is a dimeric molecule consisting of two separate subunits Band M, giving rise to three isoenzymes CK-BB, CK-MB and CK-MM. Most of the CK found in the plasma of normal individuals is the MM isoenzyme derived from skeletal muscle, while the CK-MB fraction originates predominately from cardiac muscle. Normally there is little, if any, CK-BB isoenzyme pr...

The function of creatine kinase (CK) and its effect on phosphorus metabolites was studied in livers of transgenic mice expressing human ubiquitous mitochondrial CK (CK-Mit) and rat brain CK (CK-B) isoenzymes and their combination. (31)P NMR spectroscopy and saturation transfer were recorded in livers of anesthetized mice to measure high-energy phosphates and hepatic CK activity. CK reaction vel...

Activity of creatine kinase B subunit, as measured by an immunoinhibition assay, is persistently increased in certain healthy, asymptomatic adults, whose values for total CK activity are within normal limits. Serum samples from two such individuals were investigated by electrophoresis, heat inactivation, determination of activation energies, and immunosorption (on protein A). The results demons...

A 74-year-old man with myxedema and hypothermia had increased activities in plasma of creatine kinase (CK; EC 2.7.3.2), aspartate aminotransferase (AST; EC 2.6.1.1), and lactate dehydrogenase (LD; EC 1.1.1.27) and increased proportions of CK-MB (up to 20% of total CK) and LD1 isoenzymes, but no clinical or investigational evidence of associated myocardial infarction. This case illustrates that ...

The nucleotide sequence of creatine kinase-M (CK-M) cDNA clones has been determined. It includes the entire coding region of 381 amino acids in addition to 5' and 3' untranslated regions. A comparison with a partial sequence from rat CK-M reveals 84% nucleotide sequence homology in the coding region but divergence in the 3' untranslated region. The amino acid sequence is 94% conserved between c...

The distribution of plasma creatine kinase (CK; E.C.2.7.3.2) is known to be skewed, and this has made it difficult to analyse the sources of variation. We have studied plasma CK in 206 pairs of twins and have analysed the results after separating them into what appear to be two Gaussian frequency distributions. The results in the main distribution (CK less than 300 iu/1) are apparently affected...