AIMS Ramadan is a great opportunity for scientific research due to its peculiar nature. This study was designed to investigate whether morning saliva cortisol pick can change during and after Ramadan compared to before it. MATERIALS AND METHODS A total of 30 healthy volunteer men (aged between 30 and 76 years) were asked to participate in a cross-sectional study between August 12 and Septembe...

Changes in cortisol concentration in response to exercise at 3 different intensities were quantified. Ten apparently healthy, recreationally active males participated. On 4 separate occasions, subjects were assigned a random order of 1-hour cycle ergometer bouts of exercise at 44.5 +/- 5.5%, 62.3 +/- 3.8%, and 76.0 +/- 6.0% (mean +/- SD) of VO2peak and a resting control session. Saliva samples ...

A micro-scale method is presented for measurement of cortisol. Serum or plasma, 2 microliter, is diluted with buffer and the solution heated at 60 degrees C in the presence of high-affinity antibody and [3H] cortisol. Corticosteroid-binding globulin in the serum is denatured, releasing cortisol and making it available to the antibody, which is stable during the incubation. After cooling, ammoni...

We compared results obtained by (a) chemical (spectrophotometric) and competitive protein-binding assays for serum corticosteroids, and (b) competitive protein-binding and radioimmunoassay, with and without CCI-4 extraction, for serum cortisol. The two corticosteroid methods gave identical results. Radioimmunoassay with and without a CCI-4 extraction step gave identical results for cortisol. Co...

Plasma cortisol concentration as estimated by CPB radioassay increased significantly in cattle during 4 hr of acute thermal exposure while plasma progestins did not. Changes in plasma cortisol were gradual with the onset of heat exposure and correlated significantly with rectal and environmental temperatures although the correlation was greater with rectal temperature. However, the rise and dec...

Adrenocortical insufficiency such as occurs in Addison's disease causes hyponatremia and renal tubular acidosis (RTA). Hyponatremia results from both aldosterone and cortisol insufficiency. RTA is due to aldosterone insufficiency. The involvement of cortisol in RTA is unclear. Here, we report a woman in her 70s who was admitted to our hospital with severe hyponatremia (106 mEq/l) and RTA. The p...

We investigated differences between effects of natural- and bucket-suckling methods on basal serum oxytocin (OT) and cortisol concentrations, and the effect of OT concentration on affiliative and investigative behavior of calves to a novel object. Ten Japanese Black calves, balanced with birth order, were allocated evenly to natural-suckling (NS) and bucket suckling (BS) groups. Blood samples w...

The effects of two different concentrations of halothane (0.5 or 1.5%) and two different doses of thiopentone (4.5 or 10 mg kg-1) on the plasma concentrations of cortisol, growth hormone (GH) and prolactin (PRL) were studied in response to the stress of tracheal intubation. Additionally, the effect of the intratracheal administration of 4% lignocaine spray 2 ml was investigated. The study group...

Background: MR imaging is one of the best diagnostic modalities in medicine. During an MR imaging examination, three types of field are employed to produce images. Various experimental studies have been performed about the effects of each single type of field but only few studies are available on their combination to generate MR imaging. The main objective of this research work is to study the ...

Rainbow trout (Oncorhynchus mykiss) of two lines selected for low (LR) and high (HR) cortisol stress-responsiveness were subjected to confinement for a period of 336 h. Endocrine (plasma cortisol, hepatic cortisol binding) and metabolic (plasma glucose, lactate, amino acids; hepatic glycogen and alanine aminotransferase levels) indices of stress were measured at intervals in confined and unconf...