A specialized bioreactor based on the principle of simultaneous growth and dialysis permits growth of three-dimensional (3D), multiple-cell-layer osteogenic tissue from isolated osteoblasts over long, continuous-culture intervals (tested up to 10 months with no sign of necrosis). The resulting tissue recapitulates the stages of bone development observed in vivo, including phenotypic maturation ...

Hydrogen gas (∼60% H2) was produced in a continuous flow bioreactor inoculated with heat-treated soil, and fed synthetic wastewater containing glucose (9.5 g l). The pH in the bioreactor was maintained at 5.5 to inhibit consumption of H2 by methanogens. The objective of this study was to characterize bacterial communities in the reactor operated under two different hydraulic retention times (HR...

Propionic acid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionic acid from 40 g L(-1) glycerol were 0.29 g L(-1) h(-1), 0.74 mol(PA) mol(Gly)(-1) and 20 g L(-1), respectively, ...

Isopropanol-butanol fermentation carried out by C. beijerinckii can be improved immobilizing cells in a fixed bed bioreactor. In this study, bioreactor was developed using polyurethane foams as solid support for biofilm growth. The dilution rate increased over time to follow development while avoiding solvent accumulation within the A phenomenological model designed and successfully described b...

The murine B-lymphocyte hybridoma cell line, CC9C10, was grown in serum-free continuous culture at steady-state dissolved oxygen (DO) concentrations of 10%, 50%, and 100% of air saturation in both LH Series 210 (LH) and New Brunswick Scientific (NBS) CelliGen bioreactors. All culture parameters were monitored and controlled and were nominally identical at steady state in the two bioreactors. Th...