noscopy showed diffuse mucosal edema through the entire colon, with multiple reddish erosions and contact bleeding (Fig. A). On colonoscopic biopsy, homogenous amyloid deposits were seen in the parenchyma and vessels (Fig. B, H&E, ×200), and Congo red staining for the amyloid, coupled with polarized light, demonstrated the characteristic apple-green birefringence (Fig. C, Congo red, ×400). Ches...

Methods Seventy-three patients with FMF and 39 patients with active systemic inflammation due to autoimmune or idiopathic inflammation were evaluated in our reference center for amyloidosis. Mutations in MEFV and the SAA-1 exon 3 variants were analyzed for their contribution to AA risk. Amyloidosis was detected in all cases by congo red staining of kidney or bowel biopsy specimens and AA was cl...

A metagenomic (community genomic) library consisting of 5,760 bacterial artificial chromosome clones was prepared in Escherichia coli DH10B from DNA extracted from the large-bowel microbiota of BALB/c mice. DNA inserts detected in 61 randomly chosen clones averaged 55 kbp (range, 8 to 150 kbp) in size. A functional screen of the library for beta-glucanase activity was conducted using lichenin a...

Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from clinical m...

ZnFe2O4 was fabricated by a simple solution-combustion method. The structural, optical and electronic properties are investigated by XRD, TEM, FESEM, UV-vis DRS, PL, FTIR and photocurrent measurements. The photocatalytic activity of the prepared material is studied with regard to the degradation of rhodamine B (Rh B) and Congo red under solar irradiation. The kinetic study showed that the mater...

Congo red staining is rarely the source of false positives for amyloid. On the other hand, the heat artifact due to cautery in surgical specimens adopts some hyaline features, which can mimic amyloid deposits many times. Therefore, we decided to investigate the behavior of these artifacted areas with some of the ancillary techniques that are commonly used when diagnosing amyloidosis: Congo red ...

1. Congo red injected in vivo together with uric acid gives rise to more intense and diffuse red coloring in rats, especially in the subcutaneous and peri-articular tissues, than is the case in control rats injected simply with dye. 2. Uric add added in vitro to solutions of Congo red or trypan blue increases the speed of diffusion of these dyes, both through gelatin and the animal membranes (d...

We have come to divide bacteria and viruses into filterable and nonfilterable, and we have come to think that those organisms, visible or invisible, which are smaller than the pores of our filter are filterable. That size, however, cannot be the sole criterion we have known from the behavior of certain aniline dyes.* Thus Victoria blue, a basic dye, will not pass a Berkefeld filter while Congo ...

Hepatic involvement in amyloidosis is common but obstructive jaundice is a very rare complication. Occasional patients with AL amyloidosis develop obstructive jaundice as a consequence of severe intrahepatic cholestasis, but review of the English literature reveals only 23 such cases. We report a further case of obstructive jaundice due to amyloidosis in which the diagnosis was particularly dif...

The present work has been carried out to study the mechanism of electrochemical degradation of Congo red dye. Electrolysis has been carried out in a single-chamber electrochemical cell having graphite anode and platinum cathode in the presence of KCl as supporting electrolyte under amperostatic conditions. The potential across the electrodes has been adjusted so that a constant 30 mA current pa...