The cobalamin-dependent enzyme, methionine synthetase, has been purified approximately 1000-fold to apparent homogeneity from human placenta with a 19% recovery. The final two steps of the purification utilized two different affinity columns. The first was a N5-methyltetrahydrofolate-cystamine-agarose column, and the second was a S-adenosylhomocysteine-agarose column. The enzyme was eluted from...

Antiserum against rat adipocyte intrinsic plasma membrane proteins raised in rabbits has previously been shown to mimic insulin action on rat adipocyte glucose transport activity and lipolysis (Pillion, D. J., and Czech, M. P. (1978) J. Biol. Chem 253, 3761-3764). In the present studies, extraction of lipids from adipocyte membranes previously treated with dimethylmaleic anhydride to remove ext...

concentration, which, on glacial-interglacial timescales, is involved in the regulation of atmospheric pCO2. The saturation state of the ocean with respect to calcium carbonate suggests that any dissolution of calcium carbonate takes place not in the water column but at the seafloor. On the other hand, several authors suggest that a significant part of the dissolution takes place in the upper p...

abstract strawberry is one of the most important fruit crops in the world. in this study, effect of different plant densities were studied on yield and fruit quality of strawberry 'selva' in greenhouse and laboratories of university college of agriculture and natural resources during 2002-2003. uniform stolens of strawberry were planted in holes of plastic column according to factorial in compl...

A batch reactor may be combined directly with a distillation column by distilling off the light component product in order to increase the reactor temperature or to improve the product yield of an equilibrium reaction. The same amount of the light product should be removed as the amount being formed by the reaction at any time. A linearized model has been developed which describes the process b...

Tandem mass spectrometry coupled to HPLC is the state of the art technique in proteomic research. Here we describe a highly sensitive nano liquid chromatography system (nano HPLC) for analysis of protein digests. Using preconcentration in a column-switching set-up, we were able to inject large sample volumes (< or =250 microL) without significant loss of sensitivity. The major problem with this...

Robustness of reinforced concrete (RC) structures is an ongoing challenging research topic in the engineering community. During extreme event, loss vertical load-bearing elements can activate large-deformation resisting mechanisms such as membrane and catenary actions beams floor slabs cast-in-situ RC buildings to resist gravity loads. However, few studies have been conducted for precast (PC) b...

Three male and two female patients with anorgasmia and dissociated sensory loss due to an anterior spinal cord syndrome are described. Clinical, neurophysiological and quantitative sensory evaluation revealed preservation of the large fibre dorsal column functions from the lumbosacral segments with concomitant severe dysfunction or absence of the small fibre neospinothalamic mediated functions....

We have used an off-line three-dimensional (3-D) chemical transport model (CTM) to study long-term changes in stratospheric O3. The model was run from 1977–2004 and forced by ECMWF ERA-40 and operational analyses. Model runs were performed to examine the impact of increasing halogens and additional stratospheric bromine from short-lived source gases. The analyses capture much of the observed in...