Lipoteichoic acid (LTA), extracted from Streptococcus mutans 10449 by hot aqueous phenol, was partially purified by Sepharose 6B column chromatography in 0.01 M sodium acetate, pH 6.0, containing 0.25 M sodium chloride and 0.001 M EDTA. Nucleic acid and polysaccharide were precipitated from the LTA-containing column peak by the addition of 2 volumes of chloroform-methanol (1:5). The resulting s...

AMAcetyl-,-he,osanindases A andUwere purifled to homogeneityfromhuman placenta. 1n the imt'al step of purication, thfe enzymes were adsorbed on concanavalin ASepharose 4B and etuted from, the column with a-methyl D-mannoside. Subsequent purification steps includedc DEAE-cellulose column chromn-atography, QAE-Seph%adex Ediethyl-(2-hydroxypropyl)aminoethyl-Sephadex] column chromatography1 Sephade...

INTRODUCTION Pituitary autoantibodies can be determined both in patients with pituitary disease as well as patients with autoimmune endocrine diseases. The purpose of the study was to isolate and purify pituitary autoantigen using sera of patients and the microsomal fraction of the pituitary. MATERIAL AND METHODS To isolate a pituitary autoantigen, patient sera were used, which showed a stron...

Background and objectives: The aerial parts of Echinophora platyloba are added to cheese and yoghurt for flavoring. Besides, it is used for the treatment of flatulence and as air freshener. Despite several pharmacological studies on the plant, no attempt has been made to isolate non-volatile secondary metabolites from this plant. So, phytochemical investigation seems t...

Artemisinin extracted from Artemisia annua L. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. At present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. This paper purposed to further discover new indication of Artemisia annua L. connecting with ...

We evaluated simultaneous analytical methods for organophosphorus pesticides in cruciferous vegetables by gas chromatography (GC). Firstly, 36 pesticides were selected on the basis of monitoring data (April 1996-March 2003) in Aichi Prefecture. A sample was extracted with acetonitrile and the acetonitrile layer was separated by salting-out. The extract was cleaned up with gel permeation chromat...

The four main ribonucleotides of RNA can be separated on diethylaminoethylcellulose paper sheets, with results comparable to column chromatography, by irrigation with 0.05M formic acid for 2 to 3 hours, and then reversing the direction of irrigation with 4M formic acid.

The photoprotein aequorin emits light by an intramolecular reaction when a trace of Ca2+ is added. The samples of aequorin that were purified by the conventional methods of column chromatography were separated by high-performance liquid chromatography into eight molecular forms (isoaequorins), which were designated aequorins A-H. Aequorins A, C and F were obtained in crystalline states. A wide ...