The collagenase gene was cloned from Grimontia (Vibrio) hollisae 1706B, and its complete nucleotide sequence was determined. Nucleotide sequencing showed that the open reading frame was 2,301 bp in length and encoded an 84-kDa protein of 767 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, the HEXXH motif. G....

Certain products of cultured rabbit corneal epithelium or epidermis regulate collagenase production by rabbit corneal stromal cells. Here, we examined the effects of a 20-kDa cytokine derived from cultured epithelium on collagenase expression by normal human skin fibroblasts and by cells from recessive dystrophic epidermolysis bullosa, a disease characterized by over-production of a structurall...

The authors report the case of a 65-year-old, right-hand-dominant man who had severe Dupuytren's disease with multiple cords and flexion contractures of the metacarpophalangeal and proximal interphalangeal joints of both hands and underwent repeated collagenase injections for treatment. Collagenase has been shown to be safe and effective in the treatment of Dupuytren's contractures when adminis...

Folkloric use of J. curcas latex among others are to cure tooth pain, bleeding gum and as anti-inflammatory drug. Collagenase is a neutral protease released by activated macrophage and also by fibroblasts in small amounts. The aim of this study was to evaluate the effect of J. curcas latex on collagenase released by fibroblasts. Four doses of J. curcas latex from 37.5-300 g/ml were added to 3 ...

This study was conducted to compare the correlations between free radical antioxidant activity and anti-wrinkling activity. Areca nut extracted using various concentrations of ethanol (0, 40, 80, 100%) methanol at 100 °C for 90 min. The ethanolic methanolic extracts were evaluated total phenolic content (TPC), flavonoid (TFC), activity, including capacity (TAC), FRAP, DPPH, ABTS, superoxide ani...

BACKGROUND Autotransplantation of cryopreserved ovarian cortex can be associated with a risk of cancer cell reseeding. This issue could be eliminated by grafting isolated preantral follicles. Collagenase NB6 is an enzyme produced under good manufacturing practices (GMP) in compliance with requirements for tissue engineering and transplantation in humans and thus can be used to isolate preantral...

The characteristics of the binding of pure human skin fibroblast collagenase to the in vivo form of its substrate, fibrillar collagen, has been studied under in vitro conditions. Collagenase binds tightly to reconstituted native collagen fibrils and appears to remain bound to the substrate throughout ongoing collagen degradation at 37"C, independent of subsequent dilution with buffer or the add...

p53, a tumor suppressor and a transcription factor, has been shown to transcriptionally activate the expression of a number of important genes involved in the regulation of cell growth, DNA damage, angiogenesis, and apoptosis. In a computer search for other potential p53 target genes, we identified a perfect p53 binding site in the promoter of the human type IV collagenase (also called 72-kDa g...

Phytoconstituents epigallocatechin gallate and withaferin A, found in Camellia sinensis (Kangra green tea) Withania sominifera (Ashwagandha) respectively, were explored for their binding affinity towards various enzymes involved the skin-aging process. Epigallocatechin A analyzed physiochemical properties, drug-likeness human intestinal absorptivity using Data Warrior, Molsoft SwissADME (boiled...