Protein Y (PY) is an Escherichia coli cold-shock protein which has been proposed to be responsible for the repression of bulk protein synthesis during cold adaptation. Here, we present in vivo and in vitro data which clarify the role of PY and its mechanism of action. Deletion of yfiA, the gene encoding protein PY, demonstrates that this protein is dispensable for cold adaptation and is not res...

Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) ...

Experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. Ejaculates were collected from four rams. In each session, second ejaculates (n=4) were collected in a tube containing 1 mL coating buffers which were prepared by 10, 15 or 20% egg yolk plus Tris-fructose. Samples were pooled, centrifuged and the supernatant removed in the labora...

Cold shock proteins are nucleic acid-binding proteins. They are well conserved in bacteria, animals as well as plants. Prokaryotic CSPs possess a single cold shock domain while animal CSPs are flanked by Nand C-terminal domains. Eukaryotic CSPs are commonly named Y-box proteins. Interestingly, the plants CSPs contain a C-terminal domain in addition to their N-terminal CSD. The CSPs have been re...

DNA binding protein A (DbpA) is a member of the human cold shock domain-containing protein superfamily, with known functions in cell proliferation, differentiation, and stress responses. DbpA mediates tight junction-associated activities in tubular epithelial cells, but the function of DbpA in mesangial cells is unknown. Here, we found DbpA protein expression restricted to vascular smooth muscl...

When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 degrees C or 15 degrees C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. The rate of CS7.4 production reached 13% of total protein synthesis within 1-1.5 hr after a shift to 10 degrees C and subsequently dropped to a lower basal level. Regulation of CS7.4 express...

Although many studies of ectothermic vertebrates have documented compensatory changes in cold hardiness associated with changes of season, much less attention has been paid to adjustment of physiological functions and survival limits following more acute exposure to cold. We investigated the ability of hatchling painted turtles (Chrysemys picta) to increase cold hardiness in response to brief e...