Background: Pivotal roles of Nerve growth factor (NGF) in the development and survival of both neuronal and non-neuronal cells indicate its potential for the treatment of neurodegenerative diseases. However, investigation of NGF deficits in different diseases requires the availability of properly folded human b-NGF. In previous studies bacterial expression of hNGF demonstrated the feasibility o...

xi Zusammenfassung xiii Statement of Contributions xv Acknowledgments xvii List of Symbols and Abbreviations xvii  Introduction  . Introductory Example . . . . . . . . . . . . . . . . . . . . . . . .  .. Multiobjective Problems . . . . . . . . . . . . . . . . . . .  .. Selecting the Best Solutions . . . . . . . . . . . . . . . . .  .. The Hypervolume Indicator . . . . . . . . . ...

In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our resu...

We present a quantitative method for selecting optimum configurations of computer systems. For each configuration we specify a set of requirements reflecting user’s needs. The level of satisfaction of requirements is called the global preference score. Using this indicator and the total cost of each configuration we solve the following optimization problems: (1) select a configuration which max...

Patterns of codon usage and “silent” DNA divergence suggest that natural selection discriminates among synonymous codons in Drosophila. “Preferred” codons are consistently found in higher frequencies within their synonymous families in Drosophila melanogaster genes. This suggests a simple model of silent DNA evolution where natural selection favors mutations from unpreferred to preferred codons...

A comprehensive in silico analysis of 71 species representing the different taxonomic classes and physiological genre of the domain Archaea was performed. These organisms differed in their physiological attributes, particularly oxygen tolerance and energy metabolism. We explored the diversity and similarity in the codon usage pattern in the genes and genomes of these organisms, emphasizing on t...