نتایج جستجو برای: coated spermatozoa

تعداد نتایج: 61749  

2012
Maria J. Martinez-Alborcia Anthony Valverde Inmaculada Parrilla Juan M. Vazquez Emilio A. Martinez Jordi Roca

In the present study, the impact of non-functional spermatozoa on the cryopreservation success of functional boar spermatozoa was evaluated. Fifteen sperm-rich ejaculate fractions collected from five fertile boars were frozen with different proportions of induced non-functional sperm (0--native semen sample-, 25, 50 and 75% non-functional spermatozoa). After thawing, the recovery of motile and ...

Journal: :Biology of reproduction 1996
E A Martinez J M Vazquez C Matas J Gadea M I Alonso J Roca

This study examined whether washing and preincubation of boar spermatozoa was necessary to achieve high in vitro penetration rates of pig oocytes. In experiment 1, diluted, sperm-rich fractions, stored for 24 h at 16 degrees C (stored diluted sperm) were used. After centrifugation once at 50 g for 3 min, the supernatant was concentrated at 1200 g for 3 min. The pellets were washed (1200 g for 3...

2017
Fatemeh Dehghanpour Nasim Tabibnejad Farzaneh Fesahat Fatemeh Yazdinejad Ali Reza Talebi

OBJECTIVE Sperm morphology plays an important role in infertility, especially in cases of defects in the heads of spermatozoa. Tapered-head or elongated-head spermatozoa are examples of morphological abnormalities. The aim of this study was to compare the semen parameters, levels of protamine deficiency, and frequency of apoptosis between patients with normozoospermia and those with teratozoosp...

Journal: :Reproduction, nutrition, developpement 1984
C Serres M L Kann

The motility characteristics and the behaviour of caput spermatozoa were investigated. After dilution in B2 medium the majority of spermatozoa presented head-to-head agglutination and a twisted flagellum. Only a small population of these spermatozoa developed an anarchic motility pattern. The addition of crude bovine epididymal fluid forward motility protein (FMP) to the incubation medium suppr...

Journal: :The British journal of venereal diseases 1979
A P Johnson M F Osborn

The effect of iron on the attachment of gonococci to human spermatozoa was investigated using the three iron salts, ferric chloride, ferric nitrate, and ammonium ferric citrate (AFC). Ferric chloride and ferric nitrate were found to be unsuitable for such studies because they were insoluble at physiological pH values, produced a marked decrease in the pH of unbuffered medium (Ringer's solution)...

2014
Zhao-Jia Ge Qiu-Xia Liang Yi Hou Zhi-Ming Han Heide Schatten Qing-Yuan Sun Cui-Lian Zhang

BACKGROUND The adverse effects on offspring of diabetic and/or obese mothers can be passed to the next generation. However, the mechanisms behind this are still unclear. Epigenetics may play a key role during this process. METHODS To confirm the hypothesis, we investigated the DNA methylation of several imprinted genes in spermatozoa of offspring from diabetic and/or obese mothers utilizing s...

2015
Phillip M Summers Gaurav Singhal

Problem: The sequential changes in the immunogenicity of spermatozoa in male reproductive tract and the effect of seminal vesicle secretions are long thought to act as central players in influencing immunological equilibrium in the male reproductive tract. Method of Study: Popliteal lymph nodes of mice were collected on the 8th day after sensitizing them with the testicular and epididymal sperm...

Journal: :Genetics and molecular research : GMR 2014
J J Cui Y Tian Y Liu H L Huang Y Jin

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-...

2017
Hyun Woo Gil Tae Ho Lee In-Seok Park

The aim of this study was to compare the efficacy of cryopreservation methods for ex situ conservation of spermatozoa from far eastern catfish, Silurus asotus. The spermatozoa activity index (SAI) and hatching rates were higher in spermatozoa stored in Alserver's solution than those of spermatozoa stored in glucose solution. The SAI and hatching rates in all experimental groups gradually decrea...

Journal: :Reproduction, fertility, and development 2017
S Morrow J Gosálvez C López-Fernández F Arroyo W V Holt M J Guille

There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure t...

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