نتایج جستجو برای: coated plate

تعداد نتایج: 119741  

Journal: :Journal of visualized experiments : JoVE 2009
Jeff Partridge Paula Flaherty

The hallmark of metastatic cells is their ability to invade through the basement membrane and migrate to other parts of the body. Cells must be able to both secrete proteases that break down the basement membrane as well as migrate in order to be invasive. BD BioCoat Tumor Invasion System provides cells with conditions that allow assessment of their invasive property in vitro(1,2). It consists ...

Journal: :Journal of nanoscience and nanotechnology 2014
Kilmara H G Carvalho Aluizio G Brasil Paulo E Cabral Filho Denise P L A Tenório Ana C A de Siqueira Elisa S Leite Adriana Fontes Beate S Santos

We present here a new and alternative method that uses a Fluorescence Plate Reader in a different approach, not to study protein-protein interactions, but to evaluate the efficiency of the protein bioconjugation to quantum dots (QDs). The method is based on the QDs' native fluorescence and was successfully tested by employing two different QDs-proteins conjugation methodologies, one by promotin...

Journal: :Journal of proteome research 2009
Federico Torta Matteo Fusi Carlo S Casari Carlo E Bottani Angela Bachi

Protein phosphorylation controls many cellular processes and activities. One of the major challenges in the proteomic study of phosphorylation is the enrichment of substoichiometric phosphorylated peptides from complex mixtures. Titanium dioxide (TiO2)-based chromatography is now widely applied to isolate phosphopeptides because of its efficiency and flexibility. In this study, a novel TiO2 coa...

2013
Lan Ding Ke Jia Wang Wei Wang De Feng Zhu Chao Yun Yin Jin Song Liu

A thin dielectric plate can support conventional slab modes such as leaky Fabry-Perot, guided waves and radiation modes. When the plate is coated on a metallic subwavelength disk array, it can result in new confined modes, hereby named 'disks scattering slab modes' (DSSMs). By use of a terahertz time-domain spectroscopy system, we experimentally verify the existence of two types of DSSMs in ter...

Journal: :ACS nano 2015
Qiancheng Zhao Ali K Yetisen Aydin Sabouri Seok Hyun Yun Haider Butt

Holography plays a significant role in applications such as data storage, light trapping, security, and biosensors. However, conventional fabrication methods remain time-consuming, costly, and complex, limiting the fabrication of holograms and their extensive use. Here, we demonstrate a single-pulse laser ablation technique to write parallel surface gratings and Fresnel zone plates. We utilized...

Journal: :Physical review letters 2006
Jacco H Snoeijer Giles Delon Marc Fermigier Bruno Andreotti

A solid object can be coated by a nonwetting liquid since a receding contact line cannot exceed a critical speed. In this Letter we study the dynamical wetting transition at which a liquid film gets deposited by withdrawing a vertical plate out of a liquid reservoir. It has recently been predicted that this wetting transition is critical with diverging time scales and coincides with the disappe...

Journal: :Journal of cell science 1975
L C Fowke C W Bech-Hansen O L Gamborg F Constabel

Multinucleate soybean protoplasts produced by spontaneous fusion during enzyme digestion of the cell wall initiated cell division after approximately 40 h in culture. The structure of these protoplasts during mitosis and cytokinesis was studied with both light and electron microscopes. Most nuclei did not fuse but divided synchronously. Interphase nuclei was commonly connected by short narrow n...

Journal: :Micromachines 2010
Chengzhang Li Michele H. Miller

This paper investigates the design optimization of an electrostatically actuated microcantilever resonator that operates in air. The nonlinear effects of electrostatic actuation and air damping make the structural dynamics modeling more complex. There is a need for an efficient way to simulate the system behavior so that the design can be more readily optimized. This paper describes an efficien...

Journal: :Clinical chemistry 1983
A M Smith D J Macdonald

In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted with anti-CEA conjugated to horseradish peroxidase and the activity of the pe...

Journal: :Methods in molecular biology 2005
Julie R Kikkert José R Vidal Bruce I Reisch

Particle bombardment, or biolistics, is a commonly used method for genetic transformation of plants and other organisms. Millions of DNA-coated metal particles are shot at target cells or tissues using a biolistic device or gene gun. The DNA elutes off the particles that lodge inside the cells, and a portion may be stably incorporated in the host chromosomes. A protocol for the generation of tr...

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