The need to find new EGFR inhibitors for use in combination with radiotherapy in the treatment of solid tumors has drawn our attention to compounds derived from genistein, a natural isoflavonoid. The antiproliferative potential of synthetic genistein derivatives used alone or in combination with ionizing radiation was evaluated in cancer cell lines using clonogenic assay. EGFR phosphorylation w...


 Purpose: To study the anticancer effect of naturally-occurring irigenin isoflavonoid on colon cancer, and to determine mechanism involved.
 Methods: The viability normal cancerous cells was assessed by MTT assay, while clonogenic assay used measure colony generation. Autophagy examined transmission electron microscopy (TEM) western blotting. Transwell chamber influence cell migratio...

Objective(s):P In vitro chemosensitivity and resistance assays (CSRAs) are a promising tool for personalized treatment of glioblastoma multiform (GBM). These assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. We aimed to assess the feasibility and validity of utilizing stereotactic biopsies of GBM in CSRAs. Materials and Methods: Single...

We have studied the effect of recombinant human hematopoietic growth factors (interleukin-3 [rhIL-3], granulocyte-macrophage colony-stimulating factor [rhGM-CSF], and granulocyte CSF [rhG-CSF]) on the clonal growth of human colon adenocarcinoma cell lines HTB-38, CCL 187, and WiDr (CCL 218). The factors stimulated clonal growth of HTB-38 and CCL 187 in a capillary modification of the human tumo...

Slug is associated with the radioresistance of nasopharyngeal carcinoma (NPC) and the main current approach of treatment for NPC is radiotherapy. Hence, the aim of the current study was to determine the effect of Slug silencing on the radiosensitivity of NPC cells. Lentiviral-mediated transfection of Slug RNA interference (RNAi) in NPC cell line C666-1 was performed in vitro. Following Slug inh...

Background: Tumor suppressor genes are often engaged in signaling pathways to regulate proliferation so as to block growth of tumor cells. To explore the mechanism underlying the tumor suppression activity of BLU/ZMYND10 gene on a frequently lost human chromosomal region, adenoviral vector with BLU cDNA insert was constructed. Methods: BLU gene was re-expressed in nasopharyngeal carcinoma cells...

2,5-bis(3'-Indolyl)pyrroles, analogues of the marine alkaloid nortopsentin, were conveniently prepared through a three step procedure in good overall yields. Derivatives 1a and 1b exhibited concentration-dependent antitumor activity towards a panel of 42 human tumor cell lines with mean IC50 values of 1.54 μM and 0.67 μM, respectively. Investigating human tumor xenografts in an ex-vivo clonogen...

BACKGROUND Novel therapies capable of targeting drug resistant clonogenic MM cells are required for more effective treatment of multiple myeloma. This study investigates the cytotoxicity of natural killer cell lines against bulk and clonogenic multiple myeloma and evaluates the tumor burden after NK cell therapy in a bioluminescent xenograft mouse model. DESIGN AND METHODS The cytotoxicity of...

objective(s):p in vitro chemosensitivity and resistance assays (csras) are a promising tool for personalized treatment of glioblastoma multiform (gbm). these assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. we aimed to assess the feasibility and validity of utilizing stereotactic biopsies of gbm in csras. materials and methods: single...