نتایج جستجو برای: cloned bovine embryos
تعداد نتایج: 144583 فیلتر نتایج به سال:
Aberrant epigenetic reprogramming is the main obstacle to the development of somatic cell nuclear transfer (SCNT) embryos and the generation of induced pluripotent stem (iPS) cells, which results in the low reprogramming efficiencies of SCNT and iPS. Histone H3 lysine 27 trimethylation (H3K27me3), as a repressive epigenetic mark, plays important roles in mammalian development and iPS induction....
To investigate the embryonic genome organization upon fertilization and somatic cell nuclear transfer (SCNT), we tracked HP1β and CENP, two well-characterized protein markers of pericentric and centromeric compartments respectively, in four types of embryos produced by rabbit in vivo fertilization, rabbit parthenogenesis, rabbit-to-rabbit, and bovine-to-rabbit SCNT. In the interphase nuclei of ...
interferon gamma (ifn-γ) is one of the key cytokines in defining t helper 1 lymphocyte immuneresponses. in this study, the bovine ifn-γ gene was cloned from spleen tissue rna using the reversetranscription-polymerase chain reaction (rt-pcr). ifn-γ cdna was sub-cloned and expressed inmammalian expression plasmid (pcdna3.1(+)) under the control of the human cytomegalovirus (cmv)promoter. the pred...
This review evaluates the quality of bovine embryos developed from in vitro-matured (IVM) and -fertilized (IVF) oocytes cultured in either serum-free or serum-containing media. Bovine embryos cultured in serum-supplemented medium contain numerous cytoplasmic lipid droplets and immature mitochondria compared to those cultured in serum-free medium. The accumulation of cytoplasmic lipids in embryo...
Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...
Background Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and c...
Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos. Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SO...
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