The ability to image the entire adult mouse heart at high resolution in 3-D would provide enormous advantages in the study of heart disease. However, a technique for imaging nuclear/cellular detail as well as the overall structure of the entire heart in 3-D with minimal effort is lacking. To solve this problem, we modified the benzyl alcohol:benzyl benzoate (BABB) clearing technique by labeling...

Visualization of infection and the associated host response has been challenging in adult vertebrates. Owing to their transparency, zebrafish larvae have been used to directly observe infection in vivo; however, such larvae have not yet developed a functional adaptive immune system. Cells involved in adaptive immunity mature later and have therefore been difficult to access optically in intact ...

AIM There are various techniques to study root canal morphology and diaphonization is one of them. There are various methods of decalcification and diaphonization, cited in literature and the main aim of this paper was to give a brief account of the various techniques and share our experience of the technique at a teaching institution in Karachi, Pakistan. MATERIALS AND METHODS Diaphonization...

We study three generic clearing arrangements in the presence of two-sided limited commitment: simple bilateral clearing, segregated collateral through a third party, and—most sophisticated—central counterparty (CCP) clearing. Clearing secures settlement obligations from over-the-counter forward contracts that smooth income risk-averse traders. requires to guarantee settlement; this is costly, a...

INTRODUCTION The aim of the present in vitro study was to compare the accuracy of cone-beam computed tomography (CBCT) in determining root canal morphology of mandibular first molars in comparison with staining and clearing technique. METHODS AND MATERIALS CBCT images were taken from 96 extracted human mandibular first molars and the teeth were then evaluated based on Vertucci's classificatio...

We show whether central clearing of a particular class of derivatives lowers counterparty risk. For plausible cases, adding a central clearing counterparty (CCP) for a class of derivatives such as credit default swaps reduces netting efficiency, leading to an increase in average exposure to counterparty default. Further, clearing different classes of derivatives in separate CCPs always increase...

Cellulose degradation by four cellulose-clearing brown-rot fungi in the Coniophoraceae-Coniophora prasinoides, C. puteana, Leucogyrophana arizonica, and L. olivascens-is compared with that of a non-cellulose-clearing brown-rot fungus, Poria placenta. The cellulose- and the non-cellulose-clearing brown-rot fungi apparently employ similar mechanisms to depolymerize cellulose; most likely a nonenz...